温度调节了鱼前细胞的透灵敏度
G H T Malintha1, Daniel W Woo1, Fritzie T Celino-Brady1,2
1Department of Human Nutrition, Food and Animal Sciences, University of Hawai'i at Mānoa, 1955 East-West Road, Honolulu, HI, 96822, USA.
Scientific reports
|November 19, 2023
概括
温度显著影响像鱼这样的鱼如何感知和响应不同的水盐度. 这项研究表明,透和热线索都相互作用,影响鱼类透受体中的前乳素释放和基因表达.
科学领域:
- 比较生理学比较生理学
- 内分泌学 在内分泌学.
- 环境适应 环境适应
背景情况:
- 益生菌 (Prl) 对于欧鱼的淡水适应至关重要.
- 莫桑比克鱼 (Oreochromis mossambicus) 的prl细胞作为光受体起作用,并且具有热敏性.
- 了解温度对透感应的影响是鱼类适应的关键.
研究的目的:
- 为了研究温度如何影响鱼 Prl 细胞中的透受体.
- 检查温度和盐度对Prl释放和基因表达的综合影响.
主要方法:
- 在淡水 (FW) 或海水 (SW) 中适应的鱼Prl细胞在不同的温度 (20,26,32°C) 和透度 (280,330,420mOsm/kg) 下进行了化.
- 测量了Prl异型 (Prl188,Prl177) 的释放,Prl异型的mRNA表达,Prl受体 (prlr1,prlr2) 和TRPV4通道.
- 分析了调节Prl.I.的关键转录因子的表达.
主要成果:
- 随着hyposmolality和更高的温度,Prl188的释放增加;在20°C时,Prl188的释放被抑制.
- 在SW鱼中,prl188mRNA随温度 (低度) 增加,而prl177则随温度 (高温度/度) 降低.
- 在32°C时,SW鱼的prlr1,prlr2,trpv4和转录因子表达下降,表明有补偿机制.
结论:
- 温度调节了鱼Prl细胞中的度受体.
- 热和透反应取决于适应盐度.
- 细胞机制存在,以弥补温度诱导的Prl释放的变化.
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