碳酸还原酶依赖于欧原醇和相关的醇的生物合成
Erik K R Hanko1, Kris Niño G Valdehuesa1, Koen J A Verhagen2
1Manchester Institute of Biotechnology, Faculty of Science and Engineering, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
Microbial cell factories
|November 19, 2023
概括
这项研究引入了一种高效的碳酸减少酶 (CAR) 途径,用于微生物生产有价值的芳香化合物,如醇和eugenol. 这种新的途径扩大了生物技术在制药和香料中的应用.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 自然存在的芳香化合物,如烯醇和尤根醇,在药品,口味和香水中至关重要.
- 这些化合物的传统生物合成途径依赖于CoA-依赖激活.
- 一个使用碳氧酸减少酶 (CAR) 的新型,CoA独立的途径为产生 (氧) 胺基甲中间体提供了替代方案.
研究的目的:
- 调查CAR途径的应用,用于将 (氧) 肉酸转化为 (氧) 肉醇的全细胞生物转化.
- 扩大CAR路径,用于生产多种烯和烯.
- 为了优化酶表达,以实现高效的微生物合成.
主要方法:
- 对异质表达酶的查,确定SrCAR和MsCAD2级联是最佳的.
- 采用组合方法在大肠杆菌中平衡SrCAR和MsCAD2的基因表达.
- 结合额外的酶,如烯醇乙转移酶和欧原醇合成酶,以延长该途径.
主要成果:
- 通过使用优化的SrCAR-MsCAD2级联,从酸中获得近100%的烯醇产量.
- 成功地以1.61 mM (264 mg/L) 的位数生产了欧原醇,超过了以前依赖于CoA的方法.
- 证明了各种 (氧) 肉醇和醇的生物合成,包括新型微生物产生的甲氧原醇.
结论:
- SrCAR-MsCAD2酶级联为生产各种 (氧) 醇和醇提供了一条有效的途径.
- 这种CAR途径扩大了通过微生物细胞工厂生产的增值分子库.
- 这些发现凸显了CAR途径在制药,香料和香料中生物技术应用的多功能性.
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