工程细菌对硫化进行定位,并在肠道微生理系统中对宿主产生度依赖的效应
Justin A Hayes1, Anna W Lunger1, Aayushi S Sharma2
1Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
Cell reports
|November 19, 2023
概括
工程细菌精确控制硫化 (H2S) 在一个肠上芯片模型的水平. 该系统揭示了H2S度如何影响宿主基因表达和新陈代谢,推动了肠道疾病研究.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 生物技术是生物技术.
背景情况:
- 硫化 (H2S) 是一种微生物气体,在肠道疾病中起着有争议的作用.
- 缺乏准确的H2S测量和相关的模型系统.
- 现有的模型不能完全复制人类的肠道环境.
研究的目的:
- 为控制H2S生产而设计大肠杆菌.
- 开发一种肠道微生理系统 (芯片),用于微生物与宿主共同培养.
- 为了研究H2S度对宿主细胞的影响.
主要方法:
- 经过工程设计的大肠杆菌可以在生理范围内定位H2S.
- 利用肠道微生理系统 (芯片) 进行微生物与宿主共同培养.
- 采用共聚焦显微镜实时可视化和H2S气体张力控制.
主要成果:
- 工程菌株殖民了芯片,并在2天内保持活跃.
- 在16倍的范围内,H2S的产量有所不同.
- 在宿主基因表达和新陈代谢中观察到依赖H2S的变化.
结论:
- 验证了一个用于研究微生物与宿主相互作用的新平台.
- 在肠道模型中证明了控制和测量H2S的能力.
- 提供了关于H2S在肠道健康和疾病中的机械作用的见解.
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