相关实验视频

Updated: Jul 10, 2025

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites

Published on: February 14, 2020

3种Eimeria tenella抗原的分子特征和免疫保护功效

Lei Zhang¹, Zhixuan Song¹, Peiyao Li¹

¹College of Animal Science and Technology, Guangxi University, Nanning 530004, China.

Poultry science

|November 19, 2023

概括

研究人员探索了针对鸟类菌病的新型疫苗候选人. 再组合的Eten5-B蛋白通过诱导免疫反应并提供对Eimeria tenella适度保护,显示出作为亚单元疫苗的潜力.

科学领域:

兽医寄生虫学兽医寄生虫学
免疫学免疫学免疫学
疫苗开发疫苗开发

背景情况:

由Eimeria引起的鸟类菌病是家禽行业的一个重大威胁.
目前的控制方法 (抗菌剂,活疫苗) 面临着药物耐药性和疫苗限制等挑战.
新型疫苗策略对于有效的菌病管理至关重要.

研究的目的:

描述三种Eimeria tenella蛋白质:Et-SAG14,Eten5-B和Et-PGM1. 这三种蛋白质的特征.
评估这些重组蛋白对禽类菌病的疫苗潜力.

主要方法:

蛋白质Et-SAG14,Eten5-B和Et-PGM1的特征是它们在Eimeria tenella阶段的局部化.
再组合蛋白 (rEt-SAG14,rEten5-B,rEt-PGM1) 被用于免疫.
挑战后测量了免疫反应 (IFN-γ,IL-10) 和抗菌作用 (ACI).

主要成果:

Et-SAG14和Et-PGM1局部化到杂虫和杂虫阶段;Eten5-B是细胞质的,而不是rhoptry.
用rEten5-B免疫接种提高了宿主IFN-γ和IL-10水平,显示了161.1的中度抗菌体指数 (ACI).
rEt-SAG14和rEt-PGM1免疫降低了免疫反应,并未能对E. tenella挑战 (ACI <80) 进行保护.

结论:

这种rEten5-B蛋白具有诱导对Eimeria tenella的免疫保护的潜力.
rEten5-B可以作为一个有前途的亚单元疫苗候选人,用于控制鸟类菌病.
在这项研究中,Et-SAG14和Et-PGM1作为亚单元疫苗并不有效.

关键词:

埃梅里亚天尼拉 (Eimeria tenella) 是一种植物.在IFN-γγ中.保护性有效性保护性有效性它们是重组蛋白质.

更多相关视频

Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens

Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens

Published on: February 20, 2015

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research

Published on: December 1, 2023

相关概念视频

Antigens Involved in Adaptive Immunity

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...

Antibody Structure

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...