在山羊养殖场的农场流行病学,毒性概况和耐甲基黄金葡萄球菌的分子特征
Muhammad Umar Javed1, Muhammad Ijaz1, Aneela Zameer Durrani1
1Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Microbial pathogenesis
|November 19, 2023
概括
甲素耐药黄金葡萄球菌 (MRSA) 在乳制品山羊中普遍存在,可以传染给人类. 这项研究在山羊和农场工人中发现了MRSA,强调了它由于毒性因素和抗生素耐药性而成为公共卫生威胁的潜力.
科学领域:
- 兽医微生物学 兽医微生物学
- 公共卫生 公共卫生
- 流行病学 流行病学
背景情况:
- 抗菌素耐药性 (AMR) 对兽医和公共卫生构成重大挑战.
- 甲素耐药黄金葡萄球菌 (MRSA) 是导致AMR的关键病原体.
- 了解动物-人界面的MRSA流行病学对于有效控制至关重要.
研究的目的:
- 调查山羊和相关人类MRSA的农场流行病学.
- 描述抗生素耐药性概况,毒性因子和MRSA的分子检测.
- 评估山羊和人类之间MRSA传播的可能性.
主要方法:
- 从30只山羊群中收集山羊奶和农场工作人员皮肤抽样样本.
- 使用表型和分子方法 (mecA基因) 隔离和确认S. aureus和MRSA.
- 病毒性基因分析,遗传学分析,蛋白分析和亚临床乳腺炎的风险因素评估.
主要成果:
- 黄金菌的流行率在山羊奶中为26.82%,在人类样本中为27.79%.
- 在山羊 (35.92%) 的MRSA患病率高于人类 (10.71%).
- 43.24%的局部MRSA分离物体表现出多药性耐药性 (MDR),耐氧环素,热胺素和菌素.
- MRSA分离物携带病毒性基因 (nuc,coag,icaA,icaD) 和与公共健康相关的肠毒素基因 (sec,seb,sed).
- 遗传学分析表明,来自巴基斯坦牛和水牛的MRSA与进化联系.
结论:
- 牛奶山羊中MRSA是一种新兴和流行的病原体,具有传染到人类的重大潜力.
- 在局部MRSA分离物中存在多种毒性因子和多药性耐药性,这在动物-人类界面构成威胁.
- 进一步的研究是有必要的,以解决MRSA在奶山养殖系统中的公共卫生影响.
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