穆坦皮层通过调节细胞,老鼠和斑马鱼中的凝血-炎症级联通路来发挥血液激活和抗炎作用
Yuting Huang1, Qianru Chen1, Weijie Pan1
1School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica, State Administration of Traditional Chinese Medicine (TCM), Guangzhou, 510006, China; Engineering Technology Research Center for Chinese Materia Medica Quality of Universities in Guangdong Province, Guangzhou, 510006, China.
Journal of ethnopharmacology
|November 19, 2023
概括
原始穆坦皮层 (RMC) 通过调节凝血-炎症级联表现出显著的激活血液和抗炎性质. 这项研究验证了RMC的有效性.
科学领域:
- 药理学 药理学是指药理学的学科.
- 传统中国医药 传统中国医药
- 分子生物学分子生物学
背景情况:
- 原始穆坦皮层 (Raw Moutan Cortex,简称RMC) 是一种传统的中医药,具有很长的临床使用历史.
- 它的血液冷却和血液激活作用,以及潜在的机制,正在越来越多地被调查.
- 之前的研究表明,RMC调节了凝血-炎症级联通路.
研究的目的:
- 调查RMC激活血液和抗炎作用背后的机制.
- 提供可靠的数据支持RMC的临床应用.
- 为了验证RMC在调节凝血-炎症级联中的药理疗效.
主要方法:
- 已建立的人类静脉内皮细胞 (HUVEC) 模型,用于凝血和炎症.
- 为血液热量和血液静止综合征 (BHS) 创建了老鼠模型.
- 使用斑马鱼模型用于血栓形成和炎症,采用像ELISA,RT-PCR和西方斑块等技术.
主要成果:
- 在HUVEC中,RMC抑制了凝血和炎症.
- 在BHS大鼠中,RMC降低了门温度和血液粘度,延长了凝血时间 (PT,TT,APTT).
- 在斑马鱼中,RMC限制了血栓区域并减少了炎症细胞,同时降低了关键的凝血因子和炎症标志物.
结论:
- RMC具有激活血液和抗炎作用.
- 这些效应是通过调节凝血-炎症级联通路内的基因来实现的.
- 这些发现支持RMC在治疗包括凝血和炎症在内的疾病的临床应用.
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