重组黑猩猩腺病毒表达尖端蛋白保护免受传染性支气管炎病毒
Chengyao Hou1, Ruiqi Ni1, Lijun Zhao1
1Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, People's Republic of China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, Sichuan, People's Republic of China.
International journal of biological macromolecules
|November 19, 2023
概括
一种新的腺病毒载体疫苗 (PAD-S) 有效地保护免受禽传染性支气管炎病毒 (IBV) 的感染. 这种安全且易于生产的疫苗为禽业提供了一个有前途的解决方案.
科学领域:
- 兽医病毒学 兽医病毒学
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
背景情况:
- 禽传染性支气管炎病毒 (IBV) 在全球家禽产业造成重大经济损失.
- 目前的疫苗可能在有效性或安全性方面存在局限性.
研究的目的:
- 开发一种新的,安全和有效的IBV疫苗候选人,使用复合腺病毒载体.
- 评估候选疫苗在中的免疫性和保护性疗效.
主要方法:
- 一种复制无能的黑猩猩衍生的腺病毒载体被设计成表达IBV S基因,产生PAD-S.
- 确认了复合腺病毒的产生,表征和基因表达.
- 通过眼部和鼻腔途径接种PAD-S免疫,然后接种IBV.
- 评估了免疫反应 (抗体,细胞因子) 和保护功效.
主要成果:
- PAD-S成功地产生了良好的稳定性,并证明了高效的S基因表达.
- 在中,PAD-S的眼部和鼻腔给药诱导了强烈的特定抗体和细胞因子反应.
- PAD-S没有在中复制,这表明其安全性和环保性很高.
- 用PAD-S接种疫苗提供了对IBV挑战的完整保护,减少了病毒复制,临床症状和组织损伤.
结论:
- 一种新型重组腺病毒载体疫苗 (PAD-S) 已成功开发用于IBV.
- PAD-S具有生物安全性,高保护效率和易于生产,使其成为家禽疫苗的有希望的候选疫苗.
相关概念视频
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
00:57Viral Recombination
23.5K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.5K