胆固醇晶体在固体癌症中诱导机械创伤,炎症和新血管化,如动脉样硬化
George S Abela1,2, Venkat R Katkoori2, Dorothy R Pathak3
1Department of Medicine, Division of Cardiology, Michigan State University, East Lansing, MI, USA.
American heart journal plus : cardiology research and practice
|November 20, 2023
概括
胆固醇晶体 (CCs) 在固体癌症中发现,激活像VEGF和IL-1β这样促进瘤生长的途径,类似于它们在动脉样硬化中的作用.
科学领域:
- 在瘤学瘤学.
- 心血管科学 心血管科学
- 炎症生物学 炎症生物学
背景情况:
- 癌症和动脉样硬化有共同的风险因素和炎症途径.
- 胆固醇晶体 (CCs) 通过机械损伤和炎症参与动脉样硬化病变.
- 在固体癌症中CCs的作用尚不清楚.
研究的目的:
- 研究人类固体癌症中CCs的存在和激活.
- 确定CCs是否激活癌细胞中的炎症和促进生长的途径,包括IL-1β,VEGF,CD44和Ubiquityl-Histone H2B (Ub-H2B).
- 为了比较CCs在癌症中的作用与它们在动脉样硬化中的已知作用.
主要方法:
- 使用显微镜和免疫组织化学分析11种癌症和动脉样板的瘤样本,检测CCs,自由胆固醇和IL-1β.
- 培养乳腺癌和结肠癌细胞系,有或没有CCs.
- 在CC治疗的癌细胞中,通过西斑和免疫光检测评估VEGF,CD44和Ub-H2B的表达.
主要成果:
- 与没有癌症的边缘组织相比,癌症的CC含量,分布和自由胆固醇显著更高.
- 动脉样硬化斑块与其边缘相比,也显示了较高的CC含量和胆固醇.
- CCs诱导癌细胞增加VEGF,CD44和Ub-H2B的表达,并且观察到穿孔瘤组织.
- 与边缘组织相比,在癌症中注意到IL-1β表达的增加.
结论:
- CCs存在于固体癌症中,可以诱导创伤,类似于它们在动脉样硬化中的作用.
- CCs激活细胞因子和信号通路 (VEGF,CD44,Ub-H2B),这些通路有助于癌症的生长.
- 这些发现表明CCs是动脉样硬化和癌症进展之间的潜在联系.
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