circ_0006528促进非小细胞肺癌的进展,通过菌miR-892a和调节NRAS表达来促进非小细胞肺癌的进展
Weixi Guo1, Hongming Liu1, Ming Zhong1
1Department of Thoracic Surgery, The First Affiliated Hospital of Xiamen University.
Anti-cancer drugs
|November 20, 2023
概括
循环RNAcirc_0006528通过海绵miR-892a来增加NRAS表达,促进非小细胞肺癌 (NSCLC) 的进展. 抑制circ_0006528可以抑制NSCLC的生长和侵入.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 非小细胞肺癌 (NSCLC) 的进展和耐药性受到微RNA的影响,但它们的机制尚未完全理解.
- 循环RNAs (circRNAs) 正在成为各种癌症的关键调节者,包括NSCLC.
研究的目的:
- 研究circ_0006528在NSCLC发育和耐药性的作用.
- 阐明circ_0006528通过海绵式miR-892a调节NSCLC细胞进展并影响神经母细胞瘤鼠肉瘤病毒瘤基因 (NRAS) 表达的机制.
主要方法:
- 使用PCR和RNase R处理识别circ_0006528.
- 在circ_0006528敲定后,在NSCLC细胞系 (SPCA-1和A549) 中使用MTT,Transwell和scratch试验评估细胞增殖,入侵和迁移.
- 路西法酶记者测定证实了circ_0006528,miR-892a和NRAS之间的相互作用.
- 使用miR-892a抑制剂进行功能恢复测试.
- 使用异种移植小鼠模型进行体内研究.
主要成果:
- 发现circ_0006528在NSCLC组织中与非癌细胞组织相比表达高.
- 抑制circ_0006528显著降低了NSCLC细胞的增殖和活力.
- circ_0006528直接与miR-892a结合,而miR-892a则针对NRAS.
- 抑制miR-892a扭转了circ_0006528敲击的效果,恢复了NSCLC细胞的增殖,入侵和迁移.
结论:
- circ_0006528 在NSCLC中起到关键的瘤基因作用.
- 该circ_0006528/miR-892a/NRAS轴在调节NSCLC细胞生长和入侵方面发挥着重要作用.
- 向circ_0006528可能代表NSCLC治疗的潜在治疗策略.
更多相关视频
09:45An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
Published on: May 21, 2019
8.4K
13:18Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
Published on: March 3, 2023
1.3K
相关概念视频
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Rous Sarcoma Virus (RSV) and Cancer
5.1K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.1K
