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在单细胞水平上,流行病细分特定的miRNA和mRNA调节网络
Tong Chen1, Liangyu Yao1, Wen Liu2,3
1Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Cell cycle (Georgetown, Tex.)
|November 20, 2023
概括
精子成熟过程涉及细分特异性基因调节. 这项研究确定了控制精子功能和运动的关键微RNA (miRNA) 和信使RNA (mRNA),这些RNA控制精子功能和运动.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 精子细胞需要在上成熟才能达到受精能力.
- 流行性成熟涉及复杂的分子变化,影响精子运动和功能.
- 了解细分体特异性基因表达对于破译尾膜作用至关重要.
研究的目的:
- 识别和表征在人体脊髓中的细分特异性微RNA (miRNA) 和信使RNA (mRNA) 表达模式.
- 阐明调节网络,包括转录因子 (TF) 和点基因,控制精子成熟.
- 调查与细分特异性基因表达相关的功能途径,并确定关键的调节基因.
主要方法:
- 分析了三个批量和一个单细胞RNA测序 (scRNA-seq) 数据集.
- 跨膜片段的mRNAs和miRNAs的差异表达分析 (头皮,身体,尾巴).
- 预测上游TF和下游miRNA目标,然后与DE-mRNA交叉;构建蛋白-蛋白相互作用 (PPI) 网络和miRNA-mRNA调节网络;使用qRT-PCR验证枢纽基因.
主要成果:
- 在头皮中鉴定了570个DE-mRNA和23个DE-miRNA,在体中鉴定了175个DE-mRNA和15个DE-miRNA,在尾巴中鉴定了946个DE-mRNA和12个DE-miRNA.
- 帽子中的升级基因在细胞运动路径中得到丰富,肉体在光滑肌肉路径中,尾巴在免疫路径中.
- 构建分段特定的miRNA-mRNA调节网络,并确定验证的枢纽基因,主要在精子中.
结论:
- 这项研究揭示了特定于人类外皮膜段的独特miRNA-mRNA调节网络.
- 已识别的上游TF和下游途径为细分特定的精子成熟过程提供了洞察力.
- 这些发现强调了流行性区域化在男性生育能力方面的重要性,并提出了进一步研究的目标.
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