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不同的spike-in内部控制和临床应用在中枢神经系统感染的病原体定量疗效与元基因组测序

Zhangfan Fu¹, Jingwen Ai¹, Haocheng Zhang¹

¹Department of Infectious Diseases, National Medical Center for Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, Huashan Hospital, Shanghai Medical College, Fudan University , Shanghai, China.

Microbiology spectrum

|November 20, 2023

概括

下一代元基因组测序 (mNGS) 现在可以量化感染中的病原体. 我们比较了内部控制 (Thermus thermophilus,T1菌体,人工DNA) 以准确测量中枢神经系统感染中的病原体负荷.

科学领域:

微生物学微生物学
基因组学就是基因组学.
传染性疾病传染性疾病

背景情况:

大基因组下一代测序 (mNGS) 广泛用于病原体检测.
目前的mNGS方法缺乏可靠的病原体绝对定量.
准确的病原体负载对于诊断和管理传染病至关重要.

研究的目的:

为了比较三种mNGS的内部控制 (IC) 的定量效率:Thermus thermophilus,T1菌体和人工DNA.
在中枢神经系统 (CNS) 感染中使用mNGS开发绝对病原体量化的有效策略.
通过mNGS实现同时检测病原体和负载评估.

主要方法:

针对mNGS的三种不同的内部控制 (Thermus thermophilus,T1菌体,人工DNA) 的比较分析.
开发和验证基于mNGS的绝对病原体量化策略.
开发的策略应用于中枢神经系统感染样本.

主要成果:

确定了Thermus thermophilus,T1菌体和人工DNA作为mNGS内部控制的定量效率.
在中枢神经系统感染中开发并优化了准确的病原体负载确定策略.
使用mNGS证明了同时检测和定量病原体的可行性.

结论:

开发的IC应用策略使绝对病原体定量能够在mNGS中与病原体检测一起进行.
这种方法为及时的治疗决策和中枢神经系统感染的临床预后提供了关键数据.
具有验证IC的mNGS为全面的传染病诊断提供了强大的工具.

关键词:

中枢神经系统感染感染.内部控制是指内部的控制.mNGSS 在线观看量化量化量是指量化的量.这是一个spike-in.

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