通过使用替代浸泡工艺,微型制造凝甲基酸盐/氧酸盐复合材料
Hiroki Miyajima1, Kaori Kojima2, Hiroki Touji2
1Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.
ACS biomaterials science & engineering
|November 20, 2023
概括
研究人员使用聚合凝甲基酸盐 (pGelMA) 和酸 (HAp) 创建了3D骨模型. 这种新的方法增强了细胞粘附,为先进的骨组织工程和人工骨髓应用铺平了道路.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 生物技术是生物技术.
背景情况:
- 开发模仿骨微结构的3D支架对于骨再生至关重要.
- 现有的方法往往难以复制骨复杂的等级结构.
研究的目的:
- 为了制造模拟骨微观结构的3D水凝模型.
- 使用替代浸泡工艺 (ASP) 用酸 (HAp) 修改这些模型.
- 为了评估HAp修饰对介质干细胞 (MSC) 行为的影响.
主要方法:
- 使用立体石刻法制造聚合凝甲基酸盐 (pGelMA) 线和金字塔模型.
- 通过ASP通过控制浸泡时间和周期数来修改pGelMA模型与HAp.
- 评估HAp覆盖面和修改后的脚手架上的MSC附着度.
主要成果:
- 通过ASP实现了pGelMA模型 (100-600微米宽度) 的成功HAp修改.
- 在ASP中增加的沉浸时间和周期导致HAp覆盖率更大.
- 在pGelMA-HAp支架上MSC的粘附和扩散取决于ASP条件.
结论:
- 采用两步制造方法,可以创建HAp修改的3D水凝支架.
- 这种方法促进了骨移植和生物的先进3D细胞培养系统的开发.
- 该技术有望用于制造人工骨髓和其他骨再生疗法.
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