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基于塑体基因组和45S nrDNA的超级条形编码来验证Allium ulleungense,A. microdictyon和A. ochotense的真实性

Minyoung Lee¹, Hyo Young Lee², Jong-Soo Kang¹

¹Department of Agriculture, Plant Genomics & Breeding Institute, Research Institute of Agriculture and Life Science, Forestry and Bioresources, College of Agriculture & Life Sciences, Seoul National University, Seoul, Republic of Korea.

|November 20, 2023

概括

对Allium物种的遗传分析,包括Allium ulleungense (AU) 和Allium microdictyon (AM),使用塑体和nrDNA测序确定了杂交物种. 新的分子标记物有助于区分这些有价值的山品种.

科学领域:

基因组学就是基因组学.
植物科学植物科学
分子生物学分子生物学

背景情况:

在形态上,Allium ulleungense (AU) 和Allium microdictyon (AM) 是相似的,这使得识别变得困难.
这些Allium物种是韩国的有价值的药用和食用山品种.
了解遗传多样性对于它们的种植和繁殖至关重要.

研究的目的:

为了研究AU,AM和A. ochotense (AO) 的遗传多样性.
为准确的物种和杂交识别开发分子标记物.
为了促进Allium物种的标记器辅助繁殖.

主要方法:

超级条形编码使用塑体基因组 (质体) 和核45S核核糖体DNA (nrDNA) 序列.
六个集合的测序,代表三个物种和三个种植品种.
基于确定序列变异的十个分子标记物的开发和应用.

主要成果:

在AU和AM之间的塑体和nrDNA中发现了显著的序列变异 (SNPs和InDels).
培养的收藏被分为AU类型,AM类型和假定的杂交物 (AM x AU或AU x AM).
使用已开发的标记物对49个集合的基因定型确定了五个不同的群体,包括杂交物.

结论:

超级条形编码有效地揭示了遗传多样性,并在研究的Allium物种中确定了杂交物种.
新开发的分子标记物是物种和杂交识别的宝贵工具.
这些标记物将支持Allium物种的标记物辅助分子育种计划.

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