相关实验视频

Updated: Jul 10, 2025

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

通过非洲猪瘟病毒传染细胞-细胞传播的骑乘性亡体

Peng Gao¹, Lei Zhou¹, Jiajun Wu²

¹National Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.

Proceedings of the National Academy of Sciences of the United States of America

|November 20, 2023

概括

非洲猪瘟病毒 (ASFV) 使用亡体感染和传播. 这种机制有助于病毒逃避宿主免疫反应,影响全球猪业.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

非洲猪瘟病毒 (ASFV) 在全球猪业造成重大损失.
ASFV主要感染的是巨/单细胞系的细胞.
在此之前,ASFV进入宿主细胞的机制尚不清楚.

研究的目的:

为了阐明ASFV进入和细胞传播的机制.
为了研究在ASFV感染中亡体的作用.
了解ASFV如何逃避宿主免疫反应.

主要方法:

电子显微镜和活细胞成像用于观察ASFV感染和传播.
在体外净化ASFV载体的亡体.
使用Annexin V和抗TIM4抗体进行抑制试验.
对效细胞受体TIM4的过度表达研究.

主要成果:

ASFV诱导主要猪膜巨细胞 (PAMs) 的亡,导致亡体 (ApoBDs) 的脱落.
这些带有ASFV的ApoBDs是单层的颗粒,被邻近的PAMs吞,引发二次感染.
阻断ApoBDs上的脂胺或受体细胞上的TIM4受体会抑制ASFV通过ApoBDs传播.
ASFV ApoBD介导的传播不受猪血清的影响,这表明免疫规避的机制.

结论:

ASFV利用细胞变体来有效地进行细胞间的传播.
这一策略使ASFV能够逃避宿主免疫反应,特别是抗体介导的中和.
了解这种途径对于开发有效的ASFV控制策略至关重要.

关键词:

在ASFV中,ASFV是ASFV.它们是apoptotic体.细胞对细胞的传播免疫逃避免疫逃避

更多相关视频

Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture

Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture

Published on: September 25, 2014

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Published on: October 20, 2022

相关概念视频

Phagocytosis of Apoptotic Cells

Phagocytosis of Apoptotic Cells

Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.