相关实验视频

Updated: Jul 10, 2025

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Published on: December 16, 2021

乳酸菌肠道 (Lactobacillus Intestinalis) 促使上皮细胞通过宿主-微生物视网膜酸生物合成来抑制结肠炎相关的Th17反应

Qi-Wen Wang¹, Ding-Jia-Cheng Jia², Jia-Min He¹

¹Department of Gastroenterology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, Zhejiang, 310058, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|November 20, 2023

概括

一种新型益生菌Lactobacillus intestinalis通过向Th17细胞,显示出对小鼠的性结肠炎的保护作用. 这种益生菌调节肠道上皮质免疫反应,对炎症性肠道疾病具有潜在的治疗益处.

科学领域:

胃肠病学胃肠病学
免疫学免疫学免疫学
微生物学微生物学

背景情况:

性结肠炎 (UC) 的发病与肠道微生物群密切相关.
Th17细胞在UC相关的炎症中起着至关重要的作用.

研究的目的:

在大肠炎的小鼠模型中研究Lactobacillus intestinalis (L. intestinalis) 的治疗潜力.
阐明L. intestinalis的保护作用背后的机制.

主要方法:

在小鼠中使用德克斯硫酸盐 (DSS) 诱导的大肠炎模型.
流细胞计分析T助手17 (Th17) 细胞.
对表皮SAA1/SAA2产生和视网膜酸 (RA) 生物合成的分析.
在UC患者中评估L. intestinalis的丰度.

主要成果:

L. intestinalis 显示出对DSS诱导的大肠炎具有显著的保护作用.
保护作用依赖于T细胞和IL-17A,而L. intestinalis的目标是Th17细胞.
L. intestinalis 减少了表皮SAA1/SAA2 生产,并通过ALDH和ALDH1A2 彗代谢通过RAR-α调解增加了网膜酸 (RA) 生物合成.
在UC患者中,便L. intestinalis的丰度与C/EBPA-SAA1/2-Th17轴负相关.
在缓解大肠炎方面,L. intestinalis与梅萨拉具有协同作用.

结论:

在性结肠炎中,L. intestinalis具有治疗潜力.
它的机制涉及通过减少SAA产生和增加RA生物合成来调节上皮-免疫交叉,从而抑制Th17介导的炎症.
L. intestinalis及其代谢物RA是UC治疗的有希望的候选者.

关键词:

Th17 这是一个很好的方法.大肠炎是一种大肠炎.微生物是一种微生物.网红酸是什么意思网红酸是什么意思

更多相关视频

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Published on: May 21, 2012

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Published on: April 21, 2015

相关概念视频

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...