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超声波和多声波灌对根运河微生物群落的影响:一个ex vivo研究

Ki Hong Park¹, Ronald Ordinola-Zapata¹, W Craig Noblett¹

¹Division of Endodontics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA.

International endodontic journal

|November 20, 2023

概括

在这个ex vivo研究中,GentleWave (GW) 多声灌显示了比超声波灌激活 (UIA) 更大的微生物负载减轻. 这两种方法都导致了类似的根运河微生物多样性变化在死牙.

科学领域:

牙周内科医院牙周内科医院牙周内科医院
微生物学微生物学
牙科研究牙科研究

背景情况:

根运河感染是脉死的主要原因.
有效的消毒对于内牙治疗的成功至关重要.
新的灌激活技术旨在改善微生物的消除.

研究的目的:

为了比较超声波灌激活 (UIA) 和GentleWave (GW) 多声波灌对根管微生物多样性的影响.
为了评估这些效应在一个ex vivo模型使用提取的牙与肉死.
评估最小仪器与GW灌相结合的影响.

主要方法:

提取的下牙与脉死被用于一个ex vivo模型.
在治疗前和治疗后采集样本进行微生物分析,使用qPCR和16SrRNA测序.
两个组进行了比较:UIA与6%的NaOCl和GW灌.

主要成果:

与UIA (1.6倍) 相比,GW灌显示了微生物负载的log10显著减少 (2.6倍).
无论是UIA还是GW灌,都导致了总体微生物多样性 (β多样性) 的类似变化.
观察到具体的减少:用UIA的Eubacteria和用GW灌的Prevotella.

结论:

GW多声灌实现了显著降低微生物负载,在这个ex vivo研究中表现优于UIA.
尽管在微生物负载减少方面存在差异,但UIA和GW灌导致根运河微生物多样性的变化相似.
表面微生物污染是可以忽略不计的,并没有影响研究的统计结果.

关键词:

微生物组是一个微生物组.下一代测序是下一代的测序.低化酸的使用方法

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