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Updated: Jul 10, 2025

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM
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一个新的,简化的,干燥协议,以准备 tardigrades 扫描电子显微镜

Daniele Camarda1, Edoardo Massa2, Roberto Guidetti2

  • 1Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy.

Microscopy research and technique
|November 20, 2023
PubMed
概括

引入了一种新的,具有成本效益的扫描电子显微镜 (SEM) 准备方法,用于夜动物 (水熊). 这种快速安全的协议简化了样品干燥,最大限度地减少了所需的标本,并使详细的外部形态观测成为可能.

关键词:
脱水 脱水 脱水 脱水外部形态 外部形态组织学 组织学扫描电子显微镜扫描电子显微镜分类学 分类学.

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Preparation of Samples for Electron Microscopy01:20

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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