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相关概念视频

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

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Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
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相关实验视频

Updated: Jul 10, 2025

Generation of Genetically Modified Mice through the Microinjection of Oocytes
10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

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基因工程小鼠的基因定型协议

Advait Limaye1, Kyoungin Cho2, Bradford Hall1

  • 1National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland.

Current protocols
|November 20, 2023
PubMed
概括
此摘要是机器生成的。

本指南详细介绍了识别转基因小鼠模型的方法,重点是聚合酶连锁反应 (PCR) 技术,用于传统和集群定期间隔短平行列重复 (CRISPR) 基因编辑. 它为研究环境中准确的遗传特征验证提供了必要的协议.

关键词:
这就是CRISPR/Cas9的作用.基因组编辑 基因组编辑基因型定制是基因型定制.鼠标模型 鼠标模型

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相关实验视频

Last Updated: Jul 10, 2025

Generation of Genetically Modified Mice through the Microinjection of Oocytes
10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

20.7K
Mouse Genome Engineering Using Designer Nucleases
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Mouse Genome Engineering Using Designer Nucleases

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Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
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科学领域:

  • * 遗传学和基因组学
  • * 哺乳动物模型系统
  • * 分子生物学 * 分子生物学

背景情况:

  • *实验室小鼠对于研究基因功能和人类疾病建模至关重要.
  • *像CRISPR这样的基因编辑技术的进步使得精确的小鼠基因组操纵成为可能.
  • *标准化方法对于在研究设施中一致识别转基因小鼠模型至关重要.

研究的目的:

  • * 为识别转基因小鼠模型提供全面的指导方针.
  • *详细介绍终点聚合酶链反应 (PCR) 基因定型方法.
  • * 概述用于识别使用新基因编辑技术创建的模型的策略,包括CRISPR.

主要方法:

  • *从各种小鼠组织和样本中提取基因组DNA (尾巴活检,耳孔,胚囊,精液,血液,口腔抹布).
  • * 终点聚合酶链反应 (PCR) 用于例行基因型定型.
  • *包括T7E1/Surveyor在内的CRISPR特定测定,非目标突变检测和基因敲入/删除分析.

主要成果:

  • * 建立了可靠从各种生物样本中净化DNA的协议.
  • * 证明了终点PCR对转基因小鼠常规基因定型的有效性.
  • * 展示了验证CRISPR中介编辑的方法,包括插入/删除检测和目标外分析.

结论:

  • * 标准化分子技术对于精确描述转基因小鼠模型至关重要.
  • *终点PCR仍然是基因型定型的强大方法,并补充了CRISPR编辑模型的专门测试.
  • *这些指导方针促进使用转基因小鼠进行一致和可重复的研究.