低频电磁场影响白细胞细胞系中与代谢相关的蛋白质的表达
Karolina Wójcik-Piotrowicz1, Jolanta Kaszuba-Zwoińska2, Piotr Piszczek2
1Department of Biophysics, Jagiellonian University Medical College, Łazarza street 16, 31-530 Cracow, Poland.
Environmental toxicology and pharmacology
|November 20, 2023
概括
低频磁场 (LF-MFs) 通过改变氨酸介导通路和活性氧物种 (ROS) 水平,影响白细胞活力. 这些效应取决于MF暴露和压力刺激.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
背景情况:
- 低频磁场 (LF-MF) 越来越普遍,需要了解它们的生物效应.
- 氨酸 (CaN) 介导的信号通路和活性氧物种 (ROS) 在细胞应激反应中至关重要.
研究的目的:
- 研究LF-MFs对白细胞细胞活性的影响.
- 阐明氨酸 (CaN) 中介信号和ROS在LF-MF诱导的细胞变化的作用.
主要方法:
- 对Mono Mac 6和U937细胞系暴露于LF-MFs和/或菌素 (PMC).
- 西部布洛特分析代谢蛋白质.
- 用于ROS评估的细胞染色体C减小试验.
- 流细胞计用于细胞活力评估.
主要成果:
- 单独或与PMC一起的LF-MFs以MF-依赖的方式影响了细胞活力.
- 细胞活力的变化与蛋白质表达和ROS水平相关.
- 压力刺激影响了细胞对死亡的敏感性,观察到MF特异性的生物效应.
结论:
- LF-MFs可以通过CaN介导通路和ROS调节白细胞细胞活力.
- 该研究提供了MF对不同白细胞种群的特定生物效应的实验证据.
- 了解这些机制对于评估LF-MF暴露的潜在健康影响至关重要.
更多相关视频
10:24Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
Published on: October 28, 2014
15.3K
13:40Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
Published on: July 7, 2011
18.2K
相关概念视频
01:27Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K
01:16Calmodulin-dependent Signaling
5.2K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.2K