相关实验视频

Updated: Jul 10, 2025

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

对于siRNA的高效输送的MCT纳米乳液

Marshall S Padilla¹, Montira Tangsangasaksri¹, Chih-Chun Chang¹

¹School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53705, United States of America.

Journal of pharmaceutical sciences

|November 20, 2023

概括

这项研究开发了一种使用中链甘油三酸 (MCT) 的稳定纳米乳液,以传递向Twist1的siRNA,这是癌症转移中的关键蛋白质. 在小鼠瘤模型中,纳米乳液有效降低了Twist1mRNA水平.

科学领域:

纳米技术纳米技术
癌症生物学癌症生物学
药物运输药物运输药物运输

背景情况:

Twist1蛋白在各种癌症中促进瘤转移.
对于像siRNA这样的癌症疗法,需要有效的输送系统.
中链甘油三化物 (MCT) 是FDA批准的适用于纳米乳液配方的油.

研究的目的:

开发和表征一种稳定的油在水 (O/W) 纳米乳液,用于siRNA输送.
针对Twist1,一种涉及癌症转移的蛋白质.
评估纳米乳液在降低Twist1mRNA水平的体内功效.

主要方法:

使用MCT作为疏水阶段制备一个o/w纳米乳液.
将siRNA与二氧化-3-三甲基- (DOTAP) 复合,以产生一种疏水盐.
纳米乳液颗粒大小 (140nm) 和长期稳定性 (195天) 的表征.
在小鼠瘤模型中的体内评估,以评估Twist1mRNA的减少.

主要成果:

成功制备了一个稳定的纳米乳液,平均颗粒大小为140nm.
该纳米乳液在195天内显示出长期稳定性.
在体内给药导致Twist1mRNA水平在48小时内在小鼠瘤模型中下降46%.

结论:

开发的基于MCT的纳米乳液是一种稳定有效的系统,可以提供针对Twist1.1的siRNA.
这种纳米乳液具有作为抑制癌症转移的治疗策略的潜力.
对这种输送系统的进一步研究可以推进癌症治疗选择.

关键词:

药物交付是药物交付的过程.疏水离子离子配对中链甘油三化物中链甘油三化物纳米乳液是一种纳米乳液.这就是Twist1的原因.siRNA 是一个RNA.

更多相关视频

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

相关概念视频

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...