与SINE相关的LncRNASAWPA调节了猪细胞基因组激活
Tianyao He1, Jinyu Peng1, Shu Yang1
1Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin, 150030, P. R. China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|November 20, 2023
概括
研究人员确定了SAWPA,这是一种新型的长非编码RNA,对猪胚胎发育至关重要. 削减SAWPA会通过抑制JNK-MAPK通路,在8细胞阶段停止发育.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 与逆转移子相关的长非编码RNAs (lncRNAs) 调节小鼠的植入前发育.
- 这些与逆转移素相关的lncRNAs在猪植入前发育中的功能在很大程度上仍未被探索.
研究的目的:
- 在猪早期胚胎中选与逆转移素相关的 lncRNA.
- 确定和描述参与猪胚胎发育的新型 lncRNA.
主要方法:
- 在猪胚胎中查与逆转移素相关的lncRNAs.
- 通过基因操纵识别的lncRNA (SAWPA) 的耗尽.
- 胚胎发育阶段和信号通路的分析 (JNK-MAPK).
- 研究RNA-蛋白质复合体的形成和DNA结合相互作用.
主要成果:
- 猪中SAWPA的鉴定,SAWPA是一种8细胞胚胎特异性与SINE相关的长核非编码RNA.
- 在猪胚胎中,SAWPA的耗尽导致8细胞阶段的发育停止.
- 抑制SAWPA导致抑制JNK-MAPK信号通路.
- SAWPA通过与HNRNPA1和MED8.8的RNA-蛋白质复合体在转录中作为JNK的转录因子起作用.
结论:
- SAWPA是首个在猪体中发现的与SINE相关的功能性长非编码RNA.
- SAWPA对于猪的植入前发育至关重要,通过JNK-MAPK通路调节它.
- 这一发现为哺乳动物植入前发育中的逆转移子机制提供了新的见解.
相关概念视频
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K
Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Pleiotropy
40.5K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
40.5K
Hedgehog Signaling Pathway
7.4K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.4K
siRNA - Small Interfering RNAs
16.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K


