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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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相关实验视频

Updated: Jun 29, 2026

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

用DNA条形码编码的等离子纳米结构用于基于活动的蛋白酶传感.

Subrata Pandit¹, Mark Duchow², Wilson Chao³

¹Department of Chemistry, The University of Texas at Austin, 105 E 24th St., Austin, TX 78712, USA.

Angewandte Chemie (International ed. in English)

|November 20, 2023

概括

此摘要是机器生成的。

我们开发了新的DNA条形码等离子体纳米结构,用于敏感的蛋白酶检测. 这些传感器为疾病诊断提供高特异性和多重复合能力.

关键词:

基于活动的传感.它们是DNA DNA DNA DNA.这是一种类.等离子体纳米结构蛋白质酶可以保护

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Published on: March 21, 2025

相关实验视频

Last Updated: Jun 29, 2026

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

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Published on: December 8, 2023

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

Published on: March 21, 2025

科学领域:

纳米技术纳米技术
生物化学生化学
分子生物学分子生物学

背景情况:

蛋白酶活性在各种生物过程和疾病状态中至关重要.
现有的传感器往往缺乏复杂诊断的灵敏度,特异性或多重复合能力.

研究的目的:

开发一类新的高度敏感和多重蛋白酶活性传感器.
为了证明DNA条形编码的等离子体纳米结构 (GPD) 对于检测临床相关蛋白酶的实用性.

主要方法:

黄金纳米颗粒的功能化与-DNA合物 (GPDs) 作为蛋白酶基质.
基于CRISPR/Cas12a的测定用于检测蛋白酶分裂的DNA条形码.
通过纳米粒子聚合进行色度检测,用于视觉读取.

主要成果:

与商业探测器相比,GPD实现了超过25倍的信号启动和100倍的响应.
在室温下,检测极限低至58 pM,具有高灵敏度和选择性.
成功检测SARS-CoV-2 3CL蛋白酶和卡斯帕酶3的多重检测,以及在患者衍生细胞系中视觉检测甲素B.

结论:

用DNA条形码编码的等离子体纳米结构 (GPD) 为敏感和多重蛋白质酶检测提供了一个强大的平台.
在生物传感和疾病诊断方面,GPD提供了显著的优势,包括最小的样本处理和视觉读取能力.
这项技术有望在复杂的生物样本中检测生理相关的蛋白酶度,用于临床应用.