相关实验视频

Updated: Jul 10, 2025

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Published on: June 28, 2024

细菌体工程用于改进铜离子结合

Nuriye Korkmaz¹, Sandiego Himawan^1,2, Muhammed Usman¹

¹Biosensor Group, Korea Institute of Science and Technology Europe Forschungsgesellschaft mbH, Campus E 7.1, D-66123, Saarbrücken, Germany.

Macromolecular bioscience

|November 20, 2023

概括

基因工程病毒选择性地结合铜 (II) 离子,显示出生物修复的潜力. 这些修改后的fd病毒为环境金属清洁提供了一种新的生物材料.

科学领域:

生物材料科学生物材料科学
环境生物技术环境生物技术
分子生物学分子生物学

背景情况:

丝状fd菌体是基因修饰的多功能平台.
序列可以在菌体表面进行特定的金属离子结合.
生物修复策略对于从环境中去除有毒金属物种至关重要.

研究的目的:

为了基因工程fd病毒显示选择性铜 (II) 离子结合的变体.
描述工程病毒的物理和化学特性.
评估工程病毒在铜 (II) 离子吸附中的有效性.

主要方法:

对fd病毒进行基因改造以显示变异 (H, HG, HGF, HGFA, HGFAN, HGFANV, HGFANVA).
原子力显微镜 (AFM) 和扫描电子显微镜 (SEM) 用于结构分析.
能量分散式X射线光谱 (EDX),酶链接免疫吸收试验 (ELISA),阿加罗斯凝电泳 (AGE),泽塔潜力和诱导合等离子体质谱 (ICP-MS) 用于表征和结合研究.

主要成果:

工程菌体保持了典型的丝状结构.
HGFANVA病毒形成较大的组合,并在处理后显示有选择性的Cu (II) 结合.
EDX证实了工程病毒中有机物质和Cu (II) 的存在.
与野生类型相比,ELISA和ICP-MS显示,人工病毒的Cu(II) 结合能力提高.
AGE和zeta电位分析显示了负面电荷.

结论:

经过基因工程改造的fd病毒有效地结合Cu (II) 离子.
这些基于病毒的生物材料显示出对金属污染物的生物修复有希望.
该研究提出了一种自下而上的方法,用于从工程病毒中构建功能生物材料.

关键词:

生物修复生物修复铜铜铜铜的铜.fd 菌体是一种菌体.基因工程是基因工程,是基因工程.菌体显示器可以显示菌体.

更多相关视频

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

相关概念视频

Lytic Cycle of Bacteriophages

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...

Lysogenic Cycle of Bacteriophages

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...