通过通过m6A修饰稳定CDC45mRNA,IGF2BP2促进葡萄糖溶解和肝细胞癌干的形成
1Department of Hepatobiliary Surgery, Yueyang Central Hospital, Yueyang, China.
Cell cycle (Georgetown, Tex.)
|November 20, 2023
概括
细胞分裂周期蛋白45 (CDC45) 促进肝细胞癌 (HCC) 的进展. 胰岛素样生长因子2 mRNA结合蛋白2 (IGF2BP2) 通过m6A修饰稳定了CDC45 mRNA,增强了HCC细胞生长和干性.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细胞分裂周期蛋白45 (CDC45) 已成为肝细胞癌 (HCC) 的显著预后因素.
- 了解HCC中CDC45的特定生物功能和潜在机制对于开发向疗法至关重要.
研究的目的:
- 研究CDC45在肝细胞癌 (HCC) 进展中的作用和机制.
- 探索CDC45,胰岛素样生长因子2 mRNA结合蛋白2 (IGF2BP2) 和HCC.m6A修饰之间的调控关系.
主要方法:
- 对CDC45表达和HCC的预后意义的生物信息分析.
- 在体外和体内实验涉及CDC45在HCC细胞中敲除的实验.
- RNA m6A色度测试,甲基化RNA免疫沉降 (MeRIP) 和RNA免疫沉降 (RIP) 测试.
- 通过过度表达研究评估mRNA稳定性和探索IGF2BP2的调节作用.
主要成果:
- 高的CDC45表达与HCC患者的预后不佳相关.
- CDC45 Knockdown 抑制了HCC细胞的增殖,迁移,入侵,上皮细胞-介质细胞过渡 (EMT),干性和糖解,同时促进了细胞亡.
- IGF2BP2与CDC45相互作用,其敲击降低了CDC45的mRNA稳定性.
- IGF2BP2的过度表达促进了HCC的进展,这种效应被CDC45淘汰扭转.
结论:
- 疾病预防控制中心45通过影响扩散,迁移,入侵,EMT,干性和糖解,在促进HCC进展方面发挥着关键作用.
- IGF2BP2通过m6A修饰稳定了CDC45mRNA,从而促进了HCC的糖解和干性.
- 针对IGF2BP2/CDC45轴可能为HCC提供潜在的治疗策略.
更多相关视频
08:50In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
Published on: April 18, 2025
413
06:25Author Spotlight: Assessing the Potential of Circulating Tumor Cells in Leptomeningeal Disease Research
Published on: March 29, 2024
906
相关概念视频
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K
