相关实验视频
Updated: Jul 15, 2026

06:51
Techniques to Induce and Quantify Cellular Senescence
Published on: May 1, 2017
33.4K
通过综合转录组分析识别与糖尿病足中细胞衰老相关的枢纽基因和途径
Yike Huang1, Dongqing Wang1, Wen Zhang2,3
1Department of Emergency, The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
Journal of cellular and molecular medicine
|November 20, 2023
概括
这项研究确定了糖尿病足 (DFU) 中细胞衰老 (CS) 的关键基因和途径,揭示了hsa-mir-132-3p/SIRT1/EZH2轴对DFU中衰老细胞积累至关重要.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 伤口治愈研究研究 伤口治愈研究
背景情况:
- 糖尿病足 (DFU) 是一个重大的临床挑战,细胞衰老 (CS) 与愈合受损有关.
- 了解DFU中CS的分子机制对于开发有效的治疗策略至关重要.
研究的目的:
- 在DFU中识别关键基因和与细胞衰老相关的途径.
- 阐明CS在糖尿病足病变的发病过程中的作用.
主要方法:
- 来自基因表达综合 (GEO) 数据库的GSE80178数据集的分析.
- 使用limma和CellAge. 识别差异表达基因 (DEGs) 和CS相关的DEGs (CS-DEGs).
- 基因本体学 (GO) 和基因和基因组 (KEGG) 丰富分析的京都百科全书.
- 使用String和Cytoscape构建蛋白质-蛋白质相互作用 (PPI) 网络并识别枢纽基因.
- 建立miRNA-TF-mRNA调节网络.
主要成果:
- 确定了66种CS-DEG,主要与CS相关,卡波西肉瘤相关的疹病毒感染,和Toll-like受体信号传递.
- 八个枢纽基因 (TP53,SRC,SIRT1,CCND1,EZH2,CXCL8,AR,CDK4) 被确定为DFU中衰老的调节者.
- hsa-mir-132-3p/SIRT1/EZH2轴与DFU中衰老细胞的积累有关.
结论:
- 细胞衰老在糖尿病足的病理生理学中起着重要作用.
- 特定的基因和调节网络,包括hsa-mir-132-3p/SIRT1/EZH2轴,是理解和潜在治疗DFU的关键目标.
更多相关视频
相关概念视频
Pathophysiology of Diabetes
Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility, suggesting a...
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility, suggesting a...
Diabetic Foot Ulcer
Definition A diabetic foot ulcer (DFU) is a chronic, non-healing wound that develops in individuals with diabetes. It typically occurs on pressure-bearing areas such as the heel, metatarsal heads, or hallux, and carries a high risk of infection and amputation.Pathophysiology • The development of DFUs can be explained by four interconnected mechanisms: neuropathy, ischemia, infection, and impaired wound healing. • Neuropathy is the most common factor. Sensory neuropathy reduces pain perception,...
Diabetic Nephropathy
Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration occur due to afferent arteriolar...
Diabetic Neuropathy
DefinitionDiabetic neuropathy is nerve damage caused by long-standing diabetes mellitus. It results directly from prolonged high blood sugar levels.PathophysiologyThe pathophysiology of diabetic neuropathy involves both metabolic and vascular disturbances triggered by chronic hyperglycemia.Metabolic injury: Elevated glucose levels activate the polyol pathway within nerve cells, leading to the accumulation of sorbitol and fructose. This increases oxidative stress, disrupts normal nerve...

