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一种与UVA相关的新型circuBE2I调解了HaCaT细胞中的铁亡

Peng Yi^1,2, Yan Huang^3,4, Xin Zhao^1,2

¹Department of Anesthesiology, The Affiliated Hospital, Southwest Medical University, Luzhou, Sichuan Province, China.

Photochemistry and photobiology

|November 20, 2023

概括

紫外线辐射 (UVR) 触发皮肤细胞中循环RNA (circRNA) 的变化. 研究人员将circuBE2I确定为一种关键的circRNA,它通过ferroptosis促进UVR诱导的细胞死亡,提供了一个潜在的治疗点.

科学领域:

分子生物学分子生物学
皮肤病学皮肤病学
遗传学遗传学是一个

背景情况:

预mRNA的替代拼接会产生多种异型,影响细胞命运.
紫外线辐射 (UVR) 是已知诱导皮肤细胞基因拼接变化的诱因.
紫外线诱导的循环RNA (circRNA) 在皮肤细胞中的反拼接作用在很大程度上仍未被探索.

研究的目的:

研究人类角质细胞中UVR诱导的circRNA表达特征.
为了确定参与细胞对UVA辐射反应的特定circRNAs.
探索新型circRNA,circuBE2I在UVR诱导的皮肤细胞损伤中的功能作用.

主要方法:

循环RNA转录组测序用于选UVA照射后的HaCaT细胞中差异表达的循环RNA.
用RT-qPCR验证了已识别的circRNAs的表达水平,包括circUBE2I.
RNase R消化试验证实了circuBE2I.I的稳定性.

主要成果:

在UVA暴露后,在HaCaT细胞中发现了总共312个差异表达的circRNAs.
由UBE2I基因衍生而来的新型circRNAcircuBE2I是最显著上调的circRNA.
在UVA或H2O2治疗后,circuBE2I的过度表达诱导了角质细胞中的铁亡,这种治疗被ferrostatin-1抑制.

结论:

这项研究揭示了circRNAs,特别是circUBE2I在细胞对UVA诱导损伤的反应中的重要作用.
circUBE2I促进皮肤细胞中UVR诱导的铁亡,突出显示其参与UVR相关的皮肤损伤.
circUBE2I代表了一种潜在的治疗点,用于减轻皮肤细胞中UVR诱导的铁亡.

关键词:

这就是ROSOS ROS.在UBE2I中,UBE2I是UBE2I.超紫外线 (UVA) 是一种紫外线.环RNA 环RNA 是一个环RNA.铁性化 (ferroptosis) 是一种

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