菌体vB_EfKS5的潜在应用是为了控制Enterococcus faecalis及其在食品中的生物膜
Mohamed El-Telbany1,2, Chen-Yu Lin1, Marwa Nabil Abdelaziz1
1Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.
AMB Express
|November 20, 2023
概括
一种新型的菌体,vB_EfKS5,有效地向食物中的抗生素耐药性菌体 (E. faecalis). 这种菌体显示出广泛的宿主范围和菌活性,为食品安全和公共卫生提供了有前途的解决方案.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 细菌治疗疗法是一种细菌治疗.
背景情况:
- 食品中的抗生素耐药的Enterococcus物种对公众健康构成风险.
- 在食物中积累的Enterococcus faecalis (E. faecalis) 可以导致由于生物性氨基酸而引起的细胞学影响.
- 菌体 (菌体) 是天然的抗微生物,有可能减少食品中的微生物污染物.
研究的目的:
- 为了分离一种新型细菌对E. faecalis. 有效.
- 为了确定孤立的细菌菌体的宿主范围.
- 评估菌体在食品保存中的潜在应用.
主要方法:
- 一种新型细菌菌体的分离和表征,vB_EfKS5.5.
- 基因组测序和vB_EfKS5.5的计算分析.
- 对E. faecalis和E. faecium分离物的溶解活性评估.
- 对生物膜破坏能力的评估.
- 在牛和牛奶中测试疗效,单独和与nisin一起.
主要成果:
- 体vB_EfKS5表现出广泛的宿主范围,感染了75.5%的E. faecalis和42.3%的E. faecium分离物.
- 基因组分析证实vB_EfKS5缺乏溶解性,毒性或毒性基因.
- 菌体有效地溶解了E. faecalis,并摧毁了其生物膜.
- vB_EfKS5,单独或与尼一起,控制了E. faecalis在和牛奶中的生长.
结论:
- 体vB_EfKS5是一种有前途的对抗E. faecalis. 的解毒剂.
- 其广泛的宿主范围,安全性和对生物膜的有效性支持其在食品中的应用.
- 高生产率,稳定性和快速复制周期表明食品和医疗应用的巨大潜力.
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