评估基于移动波结构的离子积累和储存,用于无损离子操纵
Pearl Kwantwi-Barima1, Sandilya V B Garimella1, Isaac K Attah1
1Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99354, United States.
Journal of the American Society for Mass Spectrometry
|November 20, 2023
概括
在无损离子操纵结构 (SLIM) 中优化离子积累需要温和的旅行波 (TW) 条件,以最大限度地减少离子损失和碎片化,特别是在空间电荷效应下. 更宽的SLIM轨道提高了离子移动性谱学的性能.
科学领域:
- 分析化学 分析化学
- 物理化学 物理化学
背景情况:
- 无损离子操纵结构 (SLIM) 提供了高分辨率的离子移动性分离和集成的离子操纵功能.
- 在SLIM中的离子积累简化了操作,并减少了对离子流动性光谱 (IMS) 外部离子陷的需求.
研究的目的:
- 描述SLIM内部的离子积累过程.
- 评估移动波 (TW) 幅度,离子收集时间和储存时间对SLIM内离子积累的影响.
主要方法:
- 使用了一个具有5个不同的轨道的SLIM模块,每个轨道都为离子积累配置.
- 研究了TW条件对3个混合物 (坎普,血管新生素II,神经新生素) 在TW速度为106m/s的作用.
- 分析了离子收集时间,储存时间和TW振幅对离子信号和损失的影响.
主要成果:
- 分析离子信号随着收集时间的增加从49到163毫秒增加,然后由于空间电荷效应,在652毫秒下降.
- 在高TW幅度 (15Vp-p和20Vp-p) 发生了显著的离子损失.
- 在空间电荷条件下,离子信号随着所有TW振幅的存储时间的增加而减少.
- 与较窄的轨道相比,更宽的SLIM轨道显示出更高的性能.
结论:
- 更温和的TW条件对于最小化SLIM内积累期间的离子损失和碎片化至关重要.
- 优化收集和储存时间对于减轻空间电荷效应和最大化离子信号至关重要.
- SLIM技术的现场积累是可行的,但需要仔细的参数优化,以有效处理离子.
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