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E 和 M SARS-CoV-2 膜蛋白表达和与植物脂质滴滴丰富
Lionel Gissot1, Florent Fontaine2, Zsolt Kelemen1
1Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Versailles, France.
Biotechnology journal
|November 21, 2023
概括
植物可以产生难以表达的疏水性蛋白质,如SARS-CoV-2 E和M.将这些蛋白质向脂滴 (LD) 有助于它们的折叠和净化,为生产植物性疫苗和药品提供了一种新方法.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 植物科学 植物科学
背景情况:
- 植物为重组蛋白质生产提供了一个可扩展的平台.
- 在植物中表达水性完整膜蛋白是具有挑战性的.
- 脂质滴 (LD) 可以促进疏水性蛋白质的折叠和净化.
研究的目的:
- 在植物中表达SARS-CoV-2 E和M整体蛋白质.
- 将这些蛋白质向脂质滴 (LDs) 以提高生产和净化.
- 评估LD针对植物系统中疏水性蛋白质表达的有效性.
主要方法:
- 在Nicotiana benthamiana和Camelina sativa中使用过渡和稳定的表达系统.
- 测试了与各种链接器和标签的蛋白质融合.
- 用三维结构预测来评估蛋白质的影响.
- 在LDs上进行了定位研究和通过浮动进行净化.
主要成果:
- 在N. benthamiana和C. sativa中表达了SARS-CoV-2的E和M蛋白.
- 虽然主要局部在N. benthamiana的ER中,但E蛋白可以在与油脂素合并时准LD.
- 在C. sativa种子中,发现E和M蛋白与纯化的LDs相关.
- 在LD中经过素介导的E和M积累的增强使得这些蛋白质在纯化分数中得到丰富.
结论:
- 将水性蛋白质 (如SARS-CoV-2 E和M) 向植物中的LD是可行的.
- 与油脂素的融合促进了LD的向和这些蛋白质的丰富.
- 该战略为在植物系统中生产和净化疏水制药和疫苗提供了一种可行的方法.
关键词:
金色的子 黄金的子这就是SARS-CoV-2病毒.卡梅琳娜·卡梅琳娜 (Camelina) 是一个在 colabfold 里面,我们把它折叠起来.脂肪液滴滴滴滴滴滴滴滴滴滴滴滴滴滴滴烟草烟草的烟草烟草是一种烟草.更多相关视频
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