iNClusive:一个数据库,收集有关非正规氨基酸及其融入蛋白质的有用信息,以更容易地实现遗传密码扩展和实施
Leon-Samuel Icking1, Andreas Martin Riedlberger1, Fabian Krause1
1Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, 79104, Freiburg, Germany.
Nucleic acids research
|November 21, 2023
概括
遗传密码的扩展使非正规氨基酸 (ncAA) 能够被纳入蛋白质中. iNClusive存储库组织了这些复杂的数据,促进了ncAA的研究和开发.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 合成生物学 合成生物学
背景情况:
- 非正规氨基酸 (ncAA) 纳入蛋白质是一个关键的生物技术.
- 遗传代码扩展 (GCE) 通过正交tRNA和合成酶促进了ncAA集成.
- 越来越多的关于ncAA整合的文献提出了数据组织的挑战.
研究的目的:
- 创建一个全面的,手动策划的ncAA合并数据库.
- 为了标准化在GCE中使用的ncAAs,tRNA合成酶 (aaRSs) 和tRNA的信息.
- 为探索ncAA整合技术的研究人员提供可访问的资源.
主要方法:
- 手动策划687个同行评审的出版物.
- 数据输入的标准化命名法.
- 专注于基于tRNA合成酶的tRNA加载,用于ncAA的整合.
- 质谱验证ncAA纳入目标蛋白质的质谱验证.
主要成果:
- iNClusive 存储库包含 2432 个条目.
- 数据包括466个ncAAs,569个蛋白标,500个aaRS和144个tRNA.
- 信息被组织和验证,重点是tRNA合成酶介导的ncAA整合.
结论:
- iNClusive为ncAA研究提供了一个有价值的,有组织的资源.
- 该存储库简化了访问有关ncAA整合方法和组件的信息.
- 预计iNClusive将加速ncAA整合技术的研究和开发.
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