通过酶性水解从狗 (Myliobatis goodei) 肝脏中提取油的比较:自由生物催化剂与固定生物催化剂
Andrés H Morales1, José H Pisa1, María I Gómez2
1Laboratorio de Biocatálisis y Biomateriales, Planta Piloto de Procesos Industriales Microbiológicos, PROIMI-CONICET, San Miguel de Tucumán, 4000, Argentina.
Journal of the science of food and agriculture
|November 21, 2023
概括
这项研究开发了一种固定化的多酶生物催化剂,用于从Myliobatis goodei肝脏中提取鱼油,获得比商业酶更高的产量 (89.97%). 这项创新支持可持续的渔业实践.
科学领域:
- 生物技术是生物技术.
- 生物催化剂是一种生物催化剂.
- 食品科学 食品科学 食品科学
背景情况:
- 提取鱼油的生物技术过程对于鱼类消费行业至关重要.
- 这项研究比较了使用自由和固定酶从Myliobatis goodei肝脏中提取油的产量.
研究的目的:
- 开发和评估一种固定化的多酶生物催化剂,用于增强鱼油提取.
- 为了比较这种新型生物催化剂与商业酶的效率.
主要方法:
- 设计了一种来自Bacillus sp.的固定生物催化剂 (Mult.Enz@MgFe2O4). 没有细胞的Mcn4提取物.
- 使用完整的因数设计优化细菌培养基,识别小麦作为对酶生产具有重要意义.
- 使用自由商用蛋白酶 (Alcalase®,Purazyme®) 和固定生物催化剂的提取产量进行比较.
主要成果:
- 固定式生物催化剂 (Mult.Enz@MgFe2O4) 实现了89.97%的优异石油提取产量.
- 商业蛋白酶Alcalase®和Purazyme®的产量分别为87.39%和84.25%.
- 提取的油具有相似的物理化学特性,尽管Purazyme®提取的油含有较低的多不和脂肪酸.
结论:
- Myliobatis goodei的副产品是提取鱼油的可行资源.
- 固定化的多酶生物催化剂在渔业中处理复杂基质方面显示出前途.
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