附录:精子核DNA碎片化,线粒体DNA碎片化和正常和异常人类射精中的拷贝数之间的关系
Ana Tímermans1,2, Fátima Otero1,2, Manuel Garrido3
1Genetics Unit, INIBIC-Complexo Hospitalario Universitario A Coruña (CHUAC), A Coruña, Spain.
Andrology
|November 21, 2023
概括
线粒体DNA碎片化和损失发生在射精后,与核DNA碎片化分开. 精子线粒体DNA拷贝数 (mtDNAcn) 可以预测非正常动物精子,而碎片化独立于精子质量.
科学领域:
- 生殖生物学 生殖生物学
- 精子体生理学 精子体生理学
- 线粒体遗传学 线粒体遗传学
背景情况:
- 人类精子核DNA碎片化 (SDF-nDNA) 动力学在射精后已知.
- 每个精子细胞中的线粒体DNA拷贝数 (mtDNAcn) 和线粒体DNA碎片化 (SDF-mtDNA) 的动态仍然没有被描述.
研究的目的:
- 为了比较mtDNAcn,SDF-mtDNA和SDF-nDNA的射精后动力学.
- 为了分析单链DNA断裂 (SDF-SSBs) 和双链DNA断裂 (SDF-DSBs) 在常态动物精子和非常态动物精子中.
- 为了研究核和线粒体DNA完整性在射精的关系.
主要方法:
- 精子样本 (常态动物精子n=28,非常态动物精子n=43) 在体外进行了0,6,24和48小时的化.
- 通过精子染色体分散 (SCD) 试验评估的SDF-nDNA.
- 使用滴滴数字PCR (dPCR) 量化mtDNAcn和SDF-mtDNA.
主要成果:
- 随着SDF-SSB和SDF-DSB的动力学,SDF-nDNA的增加.
- 非常态动物精子样本的整体核DNA碎片化速度较慢.
- 随着时间的推移,mtDNAcn线性下降,在非常态动物精子样本中是3.2倍高,并预测了非常态动物精子症.
- 在这两组中,SDF-mtDNA随着时间的推移相似地增加,最初迅速碎片化.
结论:
- 线粒体DNA碎片化和损失发生的独立于核DNA碎片化后射精.
- 精子mtDNAcn水平是预测非正常动物精子症的潜在危险因素.
- mtDNA碎片化不受精子质量的影响,这表明有不同的潜在机制.
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