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确定展开水溶性鱼蛋白的临界pH值及其对封装能力的影响

Yuying Wang¹, Jing Shen², Bowen Zou³

¹College of Food Science, Dalian Polytechnic University, Dalian 116034, China; College of Life Sciences, Key Laboratory of Biotechnology and Bioresources Utilization, Dalian Minzu University, Ministry of Education, Dalian 116600, China; State Key Laboratory of Marine Food Processing and Safety Control, China; National Engineering Research Center of Seafood, China.

Food research international (Ottawa, Ont.)

|November 21, 2023

概括

研究人员通过确定10的临界pH值,优化了使用鱼蛋白 (CP) 进行的黄素 (Cur) 封装. 这种关键的pH展开显著提高了CP-Cur复合物颗粒形成和黄素封装效率,以改善应用.

科学领域:

食品科学与技术食品科学与技术
生物化学生化学
材料科学材料科学材料科学

背景情况:

像黄素 (Cur) 这样的疏水性多具有有价值的生理活动,但由于溶解性差和不稳定性有限.
现有的pH转移方法制造鱼蛋白 (CP) -Cur复合颗粒需要高pH值,增加成本和限制应用.

研究的目的:

在pH转移过程中调查鱼蛋白 (CP) 结构展开的临界pH值.
为了确定临界pH对黄素 (Cur) 封装效率在CP-Cur复合物颗粒中的影响.

主要方法:

使用pH值转移方法制备鱼蛋白 (CP) - 黄素 (Cur) 复合颗粒.
研究了在各种pH水平上CP的结构展开,重点是临界pH.
在不同的pH值和CP:Cur摩尔比率下,量化封装效率和结合点和.

主要成果:

对于CP结构展开的关键pH值被确定为pH10,导致更大的展开.
当使用pH 10与pH 9.8.8相比,CP-Cur颗粒的封装效率增加了22.17%.
在临界pH值下,CP的结构展开增强了Cur的结合能力,和发生在较高的摩尔比率 (14对10).

结论:

在pH转移方法中使用临界pH (pH10) 对于改善鱼蛋白结构展开至关重要.
在临界pH值下优化蛋白质展开显著提高了黄素封装效率和结合能力.
这种方法为改善疏水功能物质的大规模生产和应用提供了途径.

关键词:

鱼蛋白质是鱼中的一种蛋白质.规范性变化规范性变化临界的pH值是指临界的pH值.库尔库明是一种黄素.互动互动互动互动

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