以谷物为基础的发酵食品作为叶酸和可巴胺的来源:内源微生物群的作用
Henok Ashagrie1, Kaleab Baye2, Benjamin Guibert1
1QualiSud, Université de Montpellier, Avignon Université, CIRAD, Institut Agro, IRD, Université de la Réunion, 911 avenue Agropolis, 34394 Montpellier Cedex, France.
Food research international (Ottawa, Ont.)
|November 21, 2023
概括
埃塞俄比亚的Injera发酵可以影响叶酸和可巴胺 (维生素B12) 水平,特定的细菌会影响维生素含量. 然而,发现的科巴拉明对人类来说是生物学上不活跃的.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 人类营养人类营养
背景情况:
- 叶酸和胺缺乏影响全球数百万人.
- 埃塞俄比亚的饮食依赖于英杰拉,一种发酵的谷物食品,富含叶酸,但缺乏来自动物的可巴胺.
- 像injera这样的发酵谷物产品中的可巴拉含量在很大程度上是未被探索的.
研究的目的:
- 在生产过程中评估埃塞俄比亚英杰拉的叶酸和胺含量.
- 分析影响这些维生素水平的细菌组成.
- 为了识别injera中存在的特定形式的可巴拉.
主要方法:
- 使用16S rRNA基因测序和实时PCR进行细菌组成分析.
- 使用UPLC-PDA进行科巴拉明的鉴定.
- 在不同生产阶段的内衣样本中量化叶酸和可巴胺.
主要成果:
- 令人惊的是,特夫面粉含有可巴胺,可能是微生物污染造成的.
- 在发酵过程中,叶酸和胺的含量显著变化,在后降低.
- 新鲜的injera含有高叶酸 (21.2μg/100g) 和可巴胺 (2.1μg/100g),但可巴胺在生物学上不活跃.
- 特定的细菌,如Lactobacillus coryniformis (可巴胺合成) 和Fructilactobacillus sanfranciscensis (叶酸消费),与维生素水平相关.
结论:
- 仁杰拉发酵涉及复杂的微生物相互作用,影响叶酸和科巴胺.
- 特定的细菌在发酵过程中对维生素的生产和消费起着作用.
- 使用生产维生素的细菌进行受控发酵,显示出在发酵食品中增强叶酸和科巴胺的潜力.
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