Streptomyces 全球调节者 AfsR 和 AfsS 相互作用,共同调节抗生素生产和形态发展
Yi Hao1, Wenshuai Liu1, Xingwang Li1
1State Key Laboratory of Animal Biotech Breeding and College of Biological Sciences, China Agricultural University, Beijing, China.
Microbial biotechnology
|November 21, 2023
概括
发现全球调节剂AfsR和AfsS抑制了Streptomyces avermitilis的阿弗梅克丁生产和发育. 这种相互作用增强了AfsR结合,为抗生素过度生产提供了新的方法.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 杆菌种类生产大约70%的商业抗生素.
- 全球调节剂 AfsR 和 AfsS 是已知的抗生素生产激活剂.
- 对于AfsR和AfsS,特别是AfsS的镇压作用和监管机制,目前尚不清楚.
研究的目的:
- 阐明AfsR和AfsS在Streptomyces avermitilis的avermectin生产和形态发展中的调节作用和机制.
- 研究AfsR和AfsS之间的相互作用及其对基因调节的影响.
- 为了探索其他Streptomyces物种中AfsR/AfsS相互作用的保护.
主要方法:
- 基因删除和表达分析.
- 生物化学试验包括GST拉下,微尺度热泳和共同免疫沉.
- 染色体免疫沉定量PCR (ChIP-qPCR) 来评估DNA结合亲和力.
主要成果:
- AfsR和AfsS被证明可以负面调节S. avermitilis的阿弗麦克丁生产和形态发育.
- AfsR直接抑制特定的动物基因和发育基因,同时激活其他调节基因.
- AfsS与AfsR相互作用,增强其与向促进体的结合亲和力,这种相互作用在Streptomyces物种中得到保护.
- 删除afsR或afsS显著增加了avermectin的产量.
结论:
- 在Streptomyces avermitilis中,AfsR和AfsS发挥了新的抑制作用,AfsS与AfsR一起充当共同调节剂.
- AfsR/AfsS相互作用增强了AfsR的调节功能,并且在Streptomyces中被保存.
- 了解这些调节机制为增强抗生素过度生产提供了途径.
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