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一个复杂的微流体连续流电穿孔系统,用于高效的细胞转化.

Jacob A VanderBurgh1, Grant T Corso1, Stephen L Levy1

  • 1CyteQuest, Inc.

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|November 21, 2023
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概括
此摘要是机器生成的。

本研究介绍了一种微流体电穿孔平台,用于高效的细胞重编程. 这种可适应的技术使细胞疗法的快速优化和可扩展的制造成为可能,克服了病毒载体瓶.

关键词:
转化 转化 转化 转化癌症 癌症 癌症 癌症 癌症细胞治疗疗法细胞治疗疗法电穿孔的电穿孔是一种免疫疗法 免疫疗法非病毒性非病毒性.

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科学领域:

  • 生物技术是生物技术.
  • 细胞工程 细胞工程

背景情况:

  • 细胞疗法具有广泛的疾病治疗潜力,但受到病毒载体生产瓶的阻碍.
  • 当前的电穿孔方法在产量,优化和制造的可扩展性方面面临着挑战.

研究的目的:

  • 开发一个可适应的微流体电穿孔平台,以快速,多重优化细胞遗传重编程.
  • 为了证明无的可扩展性,从小体积优化到高体积的电池制造,而无需重新优化.

主要方法:

  • 开发了一种微流体电穿孔平台,与96孔板相兼容,用于高通量选.
  • 用小细胞体积 (~20μL) 和快速测试 (每波形约3秒) 选了数百种电波形式,以检测等离子体DNA转移到Jurkat细胞中.
  • 在单独的高容量流量单元中验证优化参数,以评估可扩展性和性能.

主要成果:

  • 使用微流体平台实现了转化参数的快速,多重优化.
  • 通过优化电波形状,成功将等离子体DNA转化为Jurkat细胞.
  • 在从低容量优化扩展到高容量制造时保持了类似的转换性能.

结论:

  • 微流体平台克服了病毒载体和传统电穿孔的局限性.
  • 能够高效,成本效益和可扩展的生产细胞疗法.
  • 便于更广泛地获得先进的细胞治疗方法.