Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Complex Assembly02:41

Protein Complex Assembly

10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Synthetic Biology02:55

Synthetic Biology

4.8K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
4.8K
Mechanical Protein Functions01:58

Mechanical Protein Functions

4.9K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
4.9K
Protein Networks02:26

Protein Networks

2.3K
2.3K
Mechanical Protein Function01:58

Mechanical Protein Function

2.0K
2.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Nucleotide-Level Chemical Reaction Network Modeling Enables Quantitative Prediction of Reconstituted Cell-Free Expression Systems.

ACS synthetic biology·2026
Same author

Quantitative Measurement of Synthetic Repression Curves Reveals Design Challenges for Genetic Circuit Engineering under Growth Arrest.

ACS synthetic biology·2026
Same author

The Compositional Encoding of Hand-Eye Coordinated Movements for Single Neurons in the Posterior Parietal Cortex.

bioRxiv : the preprint server for biology·2026
Same author

Pretrained protein language models choose between sequence novelty and structural completeness.

bioRxiv : the preprint server for biology·2025
Same author

A DNA Part Library for Reliable Engineering of the Emerging Model Nematode Symbiotic Bacterium <i>Xenorhabdus griffiniae</i> HGB2511.

ACS synthetic biology·2025
Same author

ATMO: an aerially transforming morphobot for dynamic ground-aerial transition.

Communications engineering·2025

相关实验视频

Updated: Jul 10, 2025

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

9.5K

特里尔:为民主化,可扩展的蛋白质分析和工程设计编排模块化深度学习工作流程.

Zachary A Martinez1, Richard M Murray1, Matt W Thomson1

  • 1Division of Biology and Bioengineering, California Institute of Technology, Pasadena, CA 91125.

bioRxiv : the preprint server for biology
|November 21, 2023
PubMed
概括

TRILL是一个平台,通过深度学习简化了蛋白质设计和发现. 它使科学家能够通过集成先进的AI模型来创建新型蛋白质和分析生物数据,使复杂的方法更容易获得.

科学领域:

  • 计算生物学 计算生物学
  • 人工智能在生物学中的应用
  • 蛋白质工程是指蛋白质工程.

背景情况:

  • 由于大量的测序数据,深度学习模型越来越多地用于生物学.
  • 对于缺乏机器学习专业知识的研究人员来说,大型复杂的模型带来了挑战.
  • 蛋白质语言模型从广泛的序列数据中学习生物模式.

研究的目的:

  • 介绍TRILL,一个用于蛋白质设计和发现的平台.
  • 为了使科学家能够利用先进的深度学习模型用于生物应用.
  • 简化创建复杂的AI驱动蛋白质工程工作流程.

主要方法:

  • 特里尔集成了最先进的模型,如ESM-2,DifDock和RFDiffusion.
  • 它允许链接模型以创建复杂的分析和设计管道.
  • 优化的训练策略 (ZeRO-Offload,分布式数据并行) 便于在各种硬件上使用.

主要成果:

  • 特里尔可以实现新型蛋白质生成和3D结构预测.
  • 研究人员可以提取高维的蛋白质表示,并执行功能分类.
  • 该平台支持复杂的模型编排,合并各种AI功能.

更多相关视频

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

3.2K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K

相关实验视频

Last Updated: Jul 10, 2025

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

9.5K
JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

3.2K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K

结论:

  • TRILL 民主化了对生物研究中强大的深度学习工具的访问.
  • 它弥合了复杂的人工智能模型和实际科学应用之间的差距.
  • 该平台简化了生物学的先进计算方法的集成和使用.