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在基于原的接口测试中,细胞干扰是由原密度和蛋白质分解调整的

Lianne Beunk¹, Nan Wen¹, Sjoerd van Helvert¹

¹Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, GA 6525, The Netherlands.

Journal of cell science

|November 21, 2023

概括

瘤细胞的入侵取决于组织结构和矩阵金属蛋白酶 (MMP) 活性. 狭窄的空间允许通过细胞干扰进行入侵,而密集的矩阵需要MMP来进行瘤细胞迁移.

科学领域:

生物物理学的生物物理.
癌症生物学癌症生物学
生物材料是一种生物材料.

背景情况:

瘤细胞入侵涉及矩阵金属蛋白酶 (MMP) 介导的重塑和适应组织几何.
之前的3D模型探索了多孔或对齐的架构,但建筑在MMP依赖入侵中的作用仍然不清楚.

研究的目的:

为了研究不同组织架构的瘤细胞入侵疗效,模式和MMP依赖性.
为了比较入侵限制裂与密集的多孔矩阵的入侵.

主要方法:

开发一个3D接口测定与原格子之间的裂.
在模拟模型中预测细胞迁移行为.
使用MMP抑制和原降解评估进行实验验证.

主要成果:

在模型中预测了裂中的MMP独立迁移和密度矩阵中的MMP依赖迁移.
实验结果证实,抑制原体降解会影响3D原体的迁移,但不会影响接口导向迁移 (细胞阻塞).

结论:

组织结构显著影响瘤细胞入侵策略和MMP依赖性.
3D接口测定为研究相关间歇性组织拓学的入侵提供了一个模型.
这些发现可能会为针对瘤入侵的分子干预提供信息.

关键词:

蜂干扰蜂干扰蜂干扰细胞迁移细胞迁移膠原膠原裂縫模型通过ECM指导的蛋白质分解.线性监禁是一种线性监禁.瘤的球形状体

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