在金字塔神经元中以乙醇和PARP为媒介调节与核糖体相关的长非编码RNA (lncRNA)
Hooriyah S Rizavi1,2, Hannah E Gavin1, Harish R Krishnan3
1Department of Psychiatry, University of Illinois at Chicago, Chicago, IL 60612, USA.
Non-coding RNA
|November 21, 2023
概括
乙醇会改变与核糖体结合的长非编码RNA (lncRNAs),但PARP抑制剂ABT-888可以减轻这些变化. 这项研究调查了乙醇和ABT-888对lncRNA-核糖体相互作用的影响.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 长非编码RNAs (lncRNAs) 越来越多地因其多样化的细胞作用而得到认可,尽管缺乏蛋白质编码潜力.
- 一些lncRNA与核糖体结合,表明它们在翻译或转录后调节中的潜在作用.
- 已知乙醇暴露会影响细胞过程,包括基因表达和蛋白质合成.
研究的目的:
- 研究乙醇暴露对表达CaMKIIα的金字塔神经元中的与核糖体结合的lncRNAs的影响.
- 为了确定Poly-ADP рибо酶聚合酶 (PARP) 抑制剂ABT-888是否可以调节乙醇诱导的 lncRNA-核糖体协会的变化.
- 为了识别受乙醇和/或ABT-888处理影响的特定lncRNA转录.
主要方法:
- 通过腹腔内注射,小鼠接受了乙醇 (EtOH),盐水 (CTL) 或乙醇加上ABT-888 (EtOH+ABT) 的治疗.
- 从表达CaMKIIα的金字塔神经元中分离出与核糖体结合的lncRNA,使用翻译核糖体亲和力净化 (TRAP).
- 使用定量逆转录PCR (qRT-PCR) 来验证所选 lncRNA 转录的变化.
主要成果:
- 乙醇改变了107个lncRNA转录的与核糖体的关联.
- 乙醇和ABT-888的组合改变了60个lncRNA,其中49个重叠与仅乙醇的影响.
- EtOH+ABT独特地改变了11个lncRNA转录,不仅仅受到乙醇的影响,这表明PARP抑制的调节作用.
结论:
- 乙醇显著影响特定神经元群体中的核糖体结合的lncRNAs.
- PARP 抑制剂 ABT-888 可以减轻 lncRNA-核糖体相互作用中一些乙醇诱导的变化.
- 这些发现突出了一个潜在的机制,通过该机制,PARP抑制可能抵消乙醇的分子效应.
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