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牙骨液中的microRNAs:对牙周炎差异表达的观察性病例对照研究
Pedro J Almiñana-Pastor1, Francisco M Alpiste-Illueca1, Pablo Micó-Martinez1
1Department of Stomatology, Faculty of Medicine and Odontology, University of Valencia, 46010 Valencia, Spain.
Non-coding RNA
|November 21, 2023
概括
牙裂液微RNAs (miRNAs) 显示出作为牙周病生物标志物的前景. 测序确定了miR-199作为慢性牙周炎的关键指标,提供了潜在的诊断应用.
科学领域:
- 生物分子研究的研究.
- 口腔卫生诊断口腔卫生诊断口腔卫生诊断口腔卫生诊断口腔卫生诊断口腔卫生诊断口腔卫生诊断口腔卫生诊断
- 基因组学就是基因组学.
背景情况:
- 牙裂液 (GCF) 中的微RNA (miRNA) 是牙周病的潜在生物标志物.
- 慢性牙周炎是一种普遍的口腔疾病,需要准确的诊断工具.
研究的目的:
- 进行GCF.的全面miRNA测序.
- 为了比较慢性牙周炎 (CP) 患者和健康受试者 (HS) 之间的miRNA表达特征.
主要方法:
- 从严重的CP患者 (n=11) 和HS患者 (n=12) 收集的GCF样本.
- 使用miRNeasy血清/血套件分离的miRNAs.
- 通过逆转录定量聚合酶链反应 (qRT-PCR) 确定表达水平.
主要成果:
- 在CP患者GCF中,测序确定了miR-199,miR-146a,miR-30a和miR-338.
- miR-199显示出作为牙周炎生物标志物的最大潜力.
结论:
- 这项研究是第一个对GCF中所有已知的miRNA进行测序的研究.
- 确定了牙周炎的潜在的GCF衍生的miRNA生物标志物.
- 高通量测序澄清了miRNAs在牙周炎中的作用及其诊断潜力.
相关概念视频
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
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The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...