通过发酵对异烯胺进行γ-氨基化
Leonie Benninghaus1, Laura Zagami1, Giulio Tassini2
1Genetics of Prokaryotes, Faculty of Biology & CeBiTec, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany.
Chembiochem : a European journal of chemical biology
|November 21, 2023
概括
这项研究确定了第一个g-glutamyl-isopropylamide (GIPA) 的发酵性生产,这是一个有价值的中间体. 工程细菌实现了GIPA的高位数,证明了其对工业应用的潜力.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 微生物发酵 微生物发酵
背景情况:
- 谷氨基化产生N-功能化的氨基酸,而γ-谷氨基化形式提供潜在的工业效益.
- γ-Glutamyl-isopropylamide (GIPA) 是合成诸如levofloxacin和metolachlor之类的化合物的中间体.
- 目前的GIPA生产方法在工业规模上还没有成熟.
研究的目的:
- 为了建立一个强大的发酵生产方法为γ-glutamyl-isopropylamide (GIPA).
- 为高效的GIPA合成设计一个微生物宿主.
- 为了优化GIPA生产在生物反应器设置.
主要方法:
- Pseudomonas putida KT2440 的代谢工程以表达γ-glutamylmethylamide合成酶 (GMAS).
- 使用糖醇作为碳源的发酵,并添加异烯胺 (IPA).
- 优化摇瓶和料批生物反应器种植的生产.
主要成果:
- 再组合菌株在摇瓶中产生了高达21.8mM的GIPA.
- 料批生物反应器培养实现了GIPA标位11g/L.
- 获得了高产品产量 (0.11 g/g甘油) 和体积生产率 (0.24 g/L/h).
结论:
- 成功建立了第一个发酵GIPA生产工艺.
- 使用工程P. putida. 证明了GIPA的高度生产.
- 突出了微生物发酵在生产有价值的g-glutamylated化合物的潜力.
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