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Circ_0082878通过miR-455-3p/WTAP轴促进前列腺癌的进展
Pengcheng Zhao1, Pengli Han2, Yan Ma1
1Department of Urology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, China.
Environmental toxicology
|November 21, 2023
概括
循环RNAcirc_0082878通过海绵化miR-455-3p和升调WTAP促进前列腺癌 (PCa). 抑制circ_0082878抑制了PCa的生长,这表明circ_0082878/miR-455-3p/WTAP轴是潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 前列腺癌 (PCa) 是一个重要的健康问题,有未满足的治疗需求.
- 循环RNAs (circRNAs) 在PCa病变发生中的作用在很大程度上仍未被探索.
- circ_0082878在PCa中高度表达,表明其潜在的参与.
研究的目的:
- 为了研究circ_0082878在前列腺癌中的功能作用.
- 为了阐明PCa进展中circ_0082878的潜在分子机制.
- 探索circ_0082878/miR-455-3p/WTAP轴作为治疗目标的潜力.
主要方法:
- 定量实时PCR (RT-qPCR) 用于评估circ_0082878,miR-455-3p和WTAP的表达水平.
- 细胞计数工具-8 (CCK-8) 和Transwell测定用于评估细胞增殖,迁移和入侵.
- 露西法酶记者测试,RNA免疫沉降 (RIP) 和RNA拉下测试以确认分子相互作用.
- 西部斑点分析检测WTAP蛋白质水平.
- 使用小鼠模型进行体内研究,以评估circ_0082878.8.的影响.
主要成果:
- 在PCa组织和细胞中,circ_0082878和WTAP显著上调,而miR-455-3p在PCa组织和细胞中显著下调.
- 抑制circ_0082878抑制PCa细胞的增殖,迁移和入侵 in vitro和瘤生长 in vivo.
- circ_0082878作为miR-455-3p的海绵,miR-455-3p的目标是WTAP.
- circ_0082878通过降低miR-455-3p的调节,从而提高WTAP的调节,促进了PCa的进展.
结论:
- circ_0082878在前列腺癌中起着至关重要的致癌作用.
- 已识别的circ_0082878/miR-455-3p/WTAP轴代表了PCa的新型调节途径.
- 这一轴作为前列腺癌治疗的潜在治疗点具有前景.
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