14-3-3蛋白GRF8通过WRKY18-SOS通路调节果中的盐应激耐受性
Zihao Fan1,2,3,4, Yuqing Zhu1,2,3,4, Wei Kuang1,2,3,4
1College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
Plant physiology
|November 21, 2023
概括
果公司种植果植物.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 盐度是一个主要的非生物压力限制植物生长.
- 14-3-3蛋白调节植物信号通路,但它们在木质植物盐耐受性中的作用尚不清楚.
- 果 (Malus domestica) 是一种全球重要的水果作物,容易受到盐分的影响.
研究的目的:
- 为了研究14-3-3蛋白,一般调节因子8 (MdGRF8) 在果盐度耐受性中的作用.
- 阐明了MdGRF8-介导的盐应激反应背后的分子机制.
主要方法:
- 在果植物中对MdGRF8的基因表征和功能分析.
- 酵母两杂交,双分子光补充,拉下和共同免疫沉试验以确定蛋白质相互作用.
- 对MdWRKY18结合目标基因促进体 (MdSOS2,MdSOS3) 的分析.
主要成果:
- 过度表达MdGRF8增强了果的盐耐受性,而RNA干扰 (RNAi) 削弱了它.
- MdGRF8与转录因子MdWRKY18.18相互作用.
- 过度表达MdWRKY18也提高了盐分耐受性;它的沉默降低了耐受性.
- MdWRKY18直接与与盐相关的基因MdSOS2和MdSOS3.3的促进体结合.
- MdGRF8稳定了酸化的MdWRKY18,提高了其转录活性.
结论:
- MdGRF8-MdWRKY18模块在促进果的盐度应激反应方面发挥着至关重要的作用.
- 这项研究揭示了一种涉及14-3-3蛋白和WRKY转录因子的新型调节机制,用于增强木质植物的盐耐受性.
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