在肠道内P2Y和P2X纯敏受体表达的分布
Kristen A Engevik1, Francesca J Scribano1, J Thomas Gebert1
1Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States.
American journal of physiology. Gastrointestinal and liver physiology
|November 21, 2023
概括
肠上皮细胞表达纯能受体 (P2RY1,P2RY2,P2RY11,P2RX4),它们对细胞外核酸信号作出反应. 肠道有机体是研究这一关键胃肠道信号通路的有价值模型.
科学领域:
- 胃肠道学和免疫学
- 细胞生物学 细胞生物学
- 分子信号传输的方法
背景情况:
- 细胞外核酸作为信号分子,激活纯能受体 (P2RXs和P2RYs).
- 虽然在免疫和神经系统中已知P2受体在肠道炎症中的作用,但它们在肠道上皮质中的功能不明.
- 表皮细胞构成了对胃肠道刺激物和病原体的主要屏障.
研究的目的:
- 为了研究人类胃肠表皮中的P2纯能受体的表达和功能.
- 为了确定肠道上皮细胞系是否准确地模拟初级组织中观察到的纯能信号.
- 确定适合研究肠道上皮质纯能信号的模型.
主要方法:
- 来自人类蛋白质图谱和肠道细胞调查的单细胞RNA测序数据的分析.
- 在人类肠道器官和上皮细胞系中P2纯能受体表达的表征 (T84,Caco2,HT29).
- 评估通过成像对有机体和细胞系中受体激动剂的反应中的纯能信号传递.
主要成果:
- 在分析中,在胃肠道中发现了P2RY1,P2RY2,P2RY11和P2RX4的mRNA表达.
- 人体肠道有机体表现出P2RY1,P2RY2和P2RX4的突出表达,这种模式在测试的细胞系中没有保留.
- P2YR1和P2YR2激动剂在有机体中引发了强烈的反应,细胞系中反应较弱或不存在.
结论:
- 胃肠表皮表达P2RY1,P2RY2,P2RY11和P2RX4,响应细胞外纯能信号.
- 人体肠道器官精确地反映了肠道表皮中的原生纯能信号.
- 与普通细胞系相比,肠道有机体是研究表皮质纯能信号的卓越模型.
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