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植物病毒通过虫或白的实验传播

Irene Ontiveros^1,2, Juan Antonio Diaz-Pendón², Juan José López-Moya^3,4

¹Centre for Research in Agricultural Genomics (CRAG, CSIC-IRTA-UAB-UB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.

Methods in molecular biology (Clifton, N.J.)

|November 21, 2023

概括

本章详细介绍了植物病毒传播实验的标准实验室程序,使用昆虫载体,如虫和白. 它专注于优化获取,保留和注射周期,以获得可靠的结果.

科学领域:

植物病理学植物病理学
昆虫学昆虫学是一门学科.
病毒学病毒学

背景情况:

植物病毒经常由昆虫载体传播,主要是虫和白等同类动物.
成功的病毒传播取决于载体的特异性和传播方式,影响获取,保留和注射周期.

研究的目的:

描述受控,载体介导的植物病毒传播实验的基本程序.
在实验室环境中标准化实验设置,以获得可靠的结果.

主要方法:

对昆虫载体生物 (虫,白) 的处理.
管理特定的时间段,以获取,保留和接种病毒.
利用精选的植物病毒和不同的宿主植物.

主要成果:

标准化程序使得可控传输实验成为可能.
获得,保留和注射的最佳时间提高了实验可靠性.

结论:

描述的方法为可再生载体媒介病毒传播研究提供了基础.
这些程序适用于各种植物病毒,昆虫载体和宿主植物.

关键词:

收购收购收购循环传动传输系统的循环传输系统.接种方式是注射.不持久的传输不持久的传输非病毒传播媒介的非病毒传播媒介.储存储存储存储存储存半持久传输是一种半持久传输.病毒性载体病毒性载体

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