Propionibacterium freudenreichii CIRM-BIA 129通过S层蛋白B依赖的表皮增强缓解大肠炎
Marine Mantel1,2, Tony Durand1, Anne Bessard1
1The Enteric Nervous System In Gut And Brain Disorders, IMAD, Institut National de la Santé et de la Recherche Médicale, Nantes Université, Nantes, France.
使用Propionibacterium freudenreichii CIRM-BIA129可以保护小鼠免受酸 (DSS) 引起的结肠炎和肠道透. 它的保护作用由S层蛋白B介导,突出其作为肠道健康的功能性食物的潜力.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
背景情况:
- 炎症性疾病的发病率越来越高,肠道透性增加,需要功能性食物.
- 普罗皮奥尼细菌freudenreichii (P. freudenreichii) 是一种具有已知的抗炎性质的益生菌.
研究的目的:
- 在小鼠中研究P. freudenreichiiCIRM-BIA129对硫酸 (DSS) 诱导的大肠炎和肠上皮质分解的保护作用.
- 阐明S层蛋白B (SlpB) 在这些保护机制中的作用.
主要方法:
- 在DSS诱导之前,每天给小鼠服用P. freudenreichii CIRM-BIA129.
- 评估大肠炎的严重程度,肠道透性和密室长度.
- 分析了Zonula occludens-1 (ZO-1) 和中白素-1β (il-1β) 的表达.
- 在实验室中使用了Caco-2细胞模型和来自P. freudenreichii的细胞外囊泡 (EV),包括一个被删除的slpB突变.
主要成果:
- P. freudenreichii CIRM-BIA129缓解了DSS诱导的大肠炎的严重程度,并降低了肠道的通透性.
- 益生菌限制了密码损伤,增加了ZO-1表达,但没有影响il-1βmRNA水平.
- 实验室研究证实P. freudenreichii CIRM-BIA129及其EVs预防了细胞因子诱导的Caco-2单层破坏和增加TEER.
- 突变菌株或其EV中的SlpB损失取消了保护作用,并加剧了结肠炎.
结论:
- 每天摄入P. freudenreichii CIRM-BIA129可以缓解结肠炎症状,并对抗炎症诱导的肠上皮质透性.
- 保护机制涉及恢复ZO-1表达,主要由S层蛋白B介导.
- 这些发现支持使用益生菌P. freudenreichii或其后生菌分片来控制肠道透性.
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