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单组件多层自我组装蛋白质纳米粒子显示矩阵蛋白2的细胞外域作为泛流感A疫苗

Keegan Braz Gomes¹, Yi-Nan Zhang¹, Yi-Zong Lee¹

¹Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California 92037, United States.

|November 21, 2023

概括

一种具有M2e抗原的新型自组装蛋白纳米粒子 (SApNP) 疫苗提供了对流感A病毒的广泛保护. 一次剂量与强大的辅助剂在小鼠中提供了90%的保护,显示出普遍流感疫苗的前景.

科学领域:

免疫学免疫学免疫学
疫苗学疫苗学疫苗学
纳米技术纳米技术

背景情况:

开发一种交叉保护型泛型流感A疫苗是一个关键的挑战.
矩阵蛋白2 (M2e) 的保存的细胞外域是通用流感疫苗的目标.

研究的目的:

设计和评估M2e作为流感A疫苗候选人的单组件自我组装蛋白质纳米粒子 (SApNPs).
评估M2e呈现SApNP对致命流感A病毒的疗效的挑战.

主要方法:

SApNP被设计为显示来自人类,禽类和猪宿主 (M2ex3) 的M2e的并列重复.
疫苗的有效性在与H1N1和H3N2流感A病毒挑战的小鼠中进行了测试.
通过评估抗体和T细胞反应以及淋巴结中的疫苗稳定性来评估免疫性.

主要成果:

与可溶性M2e抗原相比,M2ex3SApNP显著提高了生存率并减少了体重减轻.
与辅助剂配制的SApNP显示在淋巴结中持续存在,并诱导长期存在的免疫反应.
一次低剂量的M2ex3SApNP与TLR9或STING激动剂辅助剂一起,对致命的H1N1挑战提供了90%的保护.

结论:

呈现M2ex3的SApNP是针对各种流感A菌株的有效疫苗候选者.
在SApNP平台,结合强大的辅助剂,诱导强大而持久的保护性免疫力.
这一战略有望为开发通用泛型流感A疫苗提供希望.

关键词:

M2e 的意思是M2e.一个T细胞细胞.抗体是一种抗体.甲型流感是一类流感.淋巴结的淋巴结是指淋巴结的蛋白质纳米粒子是一种蛋白质纳米粒子.疫苗疫苗疫苗疫苗

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