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相关概念视频

General Transcription Factors01:30

General Transcription Factors

5.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.3K
Transcription Factors02:16

Transcription Factors

75.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.9K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K
Lineage Commitment01:21

Lineage Commitment

3.0K
Commitment is the  process whereby stem cells:
3.0K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K

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相关实验视频

Updated: Jul 10, 2025

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
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Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

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染色体原始化元素在造血体规范之前直接组织特异性基因活性.

Alexander Maytum1, Benjamin Edginton-White1, Peter Keane1

  • 1Institute for Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.

Life science alliance
|November 21, 2023
PubMed
概括

染色体原始化是基因激活的一个关键步骤,它涉及基因表达之前开放的调节元素. 这项研究确定了这些元素,揭示了它们在发育过程中的动态,组织特异性基因控制中的作用.

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Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

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相关实验视频

Last Updated: Jul 10, 2025

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
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Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

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Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
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科学领域:

  • 发展生物学 发展生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 基因规则 基因规则

背景情况:

  • 组织特异性基因调节依赖于转录因子和表观遗传调节器在增强剂和促进剂.
  • 细胞分化是由活性增强剂模式定义的.
  • 染色体原始化,是发育基因激活的前体,仍然不太了解.

研究的目的:

  • 在胚胎干细胞分化成血液过程中,功能性地识别参与染色质原始化的增强元件.
  • 在基因表达之前激活的cis-regulatory元素的特征.
  • 为了研究信号通路在染色质原始化中的作用.

主要方法:

  • 开发了一种全基因组功能测试,用于在体外分化过程中识别增强剂.
  • 测量了全球染色质可访问性,基因组修饰和转录因子结合.
  • 综合多学科数据,以识别和描述 cis 监管要素.

主要成果:

  • 在基因表达开始之前被激活的cis-regulatory元素的识别和特征.
  • 发现一些原始元件以信号依赖的方式受到调节.
  • 证明删除一个原始元素会在发育过程中延迟相关基因的上调.

结论:

  • 发现了一个复杂的调节网络,其中早期染色体开放动态是基因表达控制的核心.
  • 强调了染色质原始化在实现动态,组织特异性基因表达方面的重要性.
  • 提供了对发育基因激活背后的分子机制的见解.