细胞外氧化物维持根表面的氧化还原活性和在大麦根尖的突然洪水引起的低氧条件下生长
Veronika Zelinová1, Loriana Demecsová1, Ľubica Liptáková1
1Institute of Botany, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovak Republic.
Planta
|November 21, 2023
概括
氧化 (NO) 有助于大麦根承受洪水压力. 应用NO捐赠剂可以保护根尖免受损伤,维持活动并防止细胞死亡,从而有助于根生长恢复.
科学领域:
- 植物生理学 植物生理学
- 环境应激反应环境应激反应
- 生物化学 生物化学
背景情况:
- 洪水引起的缺氧是植物的主要压力因素,影响根的生长和生存.
- 氧化 (NO) 与植物应激反应有关,但其在低氧耐受性方面的特定作用需要进一步研究.
- 大麦品种对缺氧压力有不同的反应.
研究的目的:
- 调查氧化 (NO) 在大麦幼苗对短期洪水引起的缺氧压力的反应中的作用.
- 为了确定NO捐赠者对根生长,氧化还原活性,脂质过氧化和细胞死亡在缺氧下的影响.
- 为了比较两个大麦品种对低氧压力的不同敏感度的反应.
主要方法:
- 两个大麦品种 (cv. 卡梅尔和cv. 在这里. 奴隶) 经历了短期的部分潜水 (低氧压力).
- 氧化捐赠者 (酸,S-nitroso-L-glutathione) 在低氧压力期间被应用.
- 测量了根生长抑制,根尖表面氧化还原活性,脂质过氧化和细胞死亡.
- 监测了根尖中的细胞外NO水平.
主要成果:
- 缺氧压力诱导了根生长停止,增加了脂质过氧化和细胞死亡. 卡梅尔,但只是降低了CV的增长率. 斯莱文,它显示出细胞外NO的升高.
- 根尖表面的氧化还原活性在两种品种的低氧下降,在CV中更快,更严重. 卡梅尔. 卡梅尔. 卡梅尔. 卡梅尔. 卡梅尔.
- 在低氧条件下,NO捐赠者的应用维持了根氧化还原活性,抑制了脂质过氧化,并部分恢复了根生长.
结论:
- 细胞外氧化 (NO) 对于在缺氧压力下维持根尖表面氧化还原活性至关重要.
- NO通过限制脂质过氧化和细胞死亡来起保护作用,从而减轻大麦中缺氧诱导的根损伤.
- 没有捐赠者的应用可以提高大麦幼苗的弹性,以短期的洪水引起的低氧压力.
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