含油的富可纳装载转基因组比格尔用于通过皮肤输送
Biswarup Das1,2, Amit Kumar Nayak3, Subrata Mallick4
1Department of Pharmaceutics, Seemanta Institute of Pharmaceutical Sciences, Mayurbhanj, Jharpokharia, Odisha, 757086, India.
AAPS PharmSciTech
|November 22, 2023
概括
这项研究开发了含有可纳的转移体大肠,以增强通过皮肤输送. 优化的配方显示持续释放和改善抗真菌活性对抗Candida albicans.
科学领域:
- 制药科学 制药科学
- 药物输送系统 药物输送系统
- 皮肤病学 皮肤病学
背景情况:
- 局部抗真菌疗法经常面临药物透和疗效方面的挑战.
- 可纳是一种广泛使用的抗真菌剂,但其局部递送需要优化.
- 转基因和大豆提供了增强通过皮肤药物递送的潜力.
研究的目的:
- 开发和优化含有可纳的转基因体大囊,用于通过皮肤输送.
- 为了研究配方变量对转移体巨特征的影响.
- 为了评估开发的巨的ex vivo通过皮浸透和抗真菌功效.
主要方法:
- 使用薄膜水化方法制备富可纳载荷转移体.
- 将转移酶体纳入一个比格尔系统.
- 使用2^3因数设计进行统计优化,分析脂质比率和边缘激活剂度.
- 转基因和巨型细胞的表征 (DEE,囊泡大小,PDI,泽塔潜力,可变形性,通过TEM/FE-SEM的形态).
- 在猪皮肤上的ex vivo透性研究.
- 对Candida albicans的抗真菌活性评估.
主要成果:
- 优化的转基因组表现出高捕获效率 (52.38%),小囊泡大小 (76.37 nm) 和良好的稳定性.
- 优化的配方 (FO) 显示了可取的物理化学性质和形态.
- 添加西瓜油 (TO-FO) 显著增强了通过皮肤透的流量 (4.101μg/cm2/h).
- 与FO大相比,TO-FO的抗真菌活性增加了1.67倍.
- 观察到转移体大的持续药物释放超过24小时.
结论:
- 统计学上优化的富可纳载体转移体大肠对于通过皮肤输送是有效的.
- 添加西瓜油可以增强透性和抗真菌功效.
- 这些转基因组合代表了真菌感染的有希望的局部输送系统.
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